干血点采样技术及其在药动学研究中的应用  

Application of dried blood spot technology in pharmacokinetic studies

作　　者：刘勇[1,2] 魏广力[1] 夏媛媛[1] 肖淑华[1] 司端运[1] 

机构地区：[1]天津药物研究院释药技术与药代动力学国家重点实验室,天津300193 [2]河南大学药学院,河南开封475000

出　　处：《药物评价研究》2011年第5期374-379,共6页Drug Evaluation Research

基　　金：国家重点基础研究发展计划项目(2010CB735602)

摘　　要：干血点采样(dried blood spot,DBS),即将全血样品收集在卡纸上,在血样采集方法上比传统方法有一定的优势。它需求较少的血量,可减少动物使用,方便血样采集、存储运输,简化样品前处理。到目前为止,DBS与LC-MS/MS技术联用在小分子定量分析中已成为一个重要的方法,将在药动学研究中得到越来越多的应用。概述DBS样品收集,储存、前处理以及分析方法。The collection of whole blood samples on paperboard, known as dried blood spot （DBS）,offers a number of advantages over conventional blood sampling.DBS requires a smaller blood volume and reduces the number of laboratory animals.It simplified sample collection,storage,shipment,and processing.To date,DBS-LC-MS/MS has emerged as an important method for quantitative analysis of small molecule drugs.It will get more and more applications in pharmacokinetic studies.This paper summarizes the collection,storage, pretreatment,and analysis for DBS samples.

关 键 词：干血点采样 LC-MS/MS 药动学 

分 类 号：R969.1[医药卫生—药理学]