黄体酮对3T3-L1脂肪前体细胞成脂分化后细胞中UCP2基因表达的影响  

Effect of Progesterone on UCP2 mRNA Expression in 3T3-L1 Adiposities

作　　者：范菲菲[1,2] 杨蕾[1,2] 冯钟徽[1,2] 陶乐[1,2] 蔡国平[1,2] 

机构地区：[1]清华大学生命科学院,北京100084 [2]清华大学深圳研究生院生命学部,广东深圳518055

出　　处：《现代生物医学进展》2011年第22期4201-4204,共4页Progress in Modern Biomedicine

摘　　要：目的:观察体外培养条件下3T3-L1脂肪前体细胞诱导分化成的成熟脂肪细胞中解偶联蛋白2(UCP2)mRNA表达水平及黄体酮对其表达的影响。方法:体外培养3T3-L1脂肪细胞,在诱导3T3-L1脂肪细胞分化成熟后,经不同黄体酮浓度10μm/25μM/50μM/75μM/100μM刺激后,抽提总RNA,用RT-PCR检测UCP2 mRNA的表达。结果:黄体酮会促进成熟脂肪细胞中UCP2 mRNA的表达,(P<0.05)其中25μM浓度刺激下UCP2 mRNA表达量最高。结论:体外培养中,黄体酮对成熟脂肪细胞中UCP2 mRNA的表达与调控具有一定的影响。Objective： To investigate the effect of glucose concentration on the expression of Uncoupling Protein 2 （UCP2） in 3T3 - L1 adipocytes. Methods： The differentiated 3T3-L1 adipocytes were incubated with 10 μM,25 ixM,50 μM,75 μM, 100 μM proges- terone. The total cellular RNA was extracted, and the expression of UCP2 mRNA were detected by RT- PCR. Results： Stimulation of progesterone increased the expression ofUCP2 mRNA （P〈0.05）, while 25μM has obvious effect, compared with that in control group. Conclusion： The progesterone is a very impact factor for the regulation ofUCP2 mRNA expression.

关 键 词：3T3-L1脂肪前体细胞 解偶联蛋白2(UCP2) 黄体酮 

分 类 号：R339.6[医药卫生—人体生理学] Q21[医药卫生—基础医学]