顺铂诱导非洲绿猴肾细胞凋亡模型的建立  

Establish Vero Cells Apoptosis Model Induced by Cisplatin

作　　者：浦洋[1] 薛礼长[1,2] 吕芳彪[1,2] 樊建勇[2] 张发洲[1,2] 龙朝钦[2] 

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510006 [2]广州军区广州总医院皮肤科,广东广州510010

出　　处：《现代生物医学进展》2011年第22期4205-4208,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金资助项目(30972666);国家自然科学基金资助项目(81071401);全军医学科学技术研究"十一五"计划资助项目(06J008)

摘　　要：目的:建立常见凋亡诱导剂顺铂(Cisplatin)诱导非洲绿猴肾细胞(Vero)凋亡的模型,为进一步研究抗凋亡基因在细胞凋亡中的分子机理打下基础。方法:分别以不同浓度的顺铂处理Vero细胞48h,用噻唑蓝(MTT)比色法、Gimesa染色、流式细胞术检测,观察处理后细胞的生长活力和凋亡情况。结果:以未处理的细胞作为对照组,1、2、3、4、5μg/mL的顺铂处理的Vero细胞生存率分别为(79.02±6.10)%、(68.84±4.42)%、(56.66±4.07)%、(46.83±3.76)%、(29.04±5.93)%(P<0.01);经顺铂诱导后细胞形态学发生明显改变,出现膜小泡和凋亡小体形成等凋亡细胞特征;流式细胞仪检测,0、1、2、3、4、5μg/mL的顺铂处理的Vero细胞后凋亡率分别为1.66%±0.19%、16.65%±1.26%、24.82%±1.03%、36.22%±1.04%、48.49%±1.24%、43.34%±1.17%(P<0.01)。结论:本实验成功建立顺铂诱导非洲绿猴肾细胞凋亡模型,将有助于进一步探讨目的基因在Vero细胞凋亡作用的的分子机制。Objective： Creat African green monkey kidney cells （Vero） apoptosis model which was induced by typical apoptosis inducer-Cisplatin （Cisplatin）, to lay a foundation for the further study of the molecular mechanism of antiapoptotic gene in cell apoptosis. Method： Vero cells were treated with cisplatin in different concentrations for 48 hours, then cell growth viability and apoptotic rate were measured by MTT, Gimesa, and flow cytometry. Results： The untreated cells was used as control group, the growth rate of cells treated by the 1,2, 3, 4, 5μ g/mL Cisplatin were （79.02± 6.10）%, （68.84± 4.42）%, （56.66± 4.07）%, （46.83± 3.76）%, （29.04± 5.93）%（P〈0.01 ） respectively. The morphology of the vero cell apparently changed and the typical characteristic of apoptotic cells, for instance, membrane vesicles and apoptotic body was appeared after treated by cisplatin; Flow cytometry was used to detect different doses of cisplatin（0, 1, 2, 3, 4, 5 μg/mL ）induced apoptosis in Vero cells after 48h, the apoptosis rates were 1.66%± 0.19%, 16.65%± 1.26%, 24.82%± 1.03%, 36.22% ±1.04%, 48.49%± 1.24%, 43.34% ± 1.17% （P〈0.01 ）respectively. Conclusion： Cisplatin-induced apoptosis model in African green monkey kidney cells were successful established which will help to fiu＇ther explore the molecular mechanisms of the role of the gene of Interest in the apoptosis in Vero cells.

关 键 词：非洲绿猴肾细胞 细胞凋亡 顺铂 凋亡模型 

分 类 号：Q95-3[生物学—动物学] Q75