艾蒿提取液对茶尺蠖生物活性的初步研究  

Biological Activities of Extracts from Artemisia argyi Against Ectropis obliqua

作　　者：孙钦玉[1] 高旭晖[1] 杨云秋[1] 袁争[1] 张亮[1] 朱尉[1] 

机构地区：[1]安徽农业大学/农业部茶叶生物化学与分子生物学重点实验室,合肥230036

出　　处：《中国农学通报》2011年第25期77-81,共5页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目"茶树对茶尺蠖危害诱导防御反应的分子机制及抗性相关基因的筛选"(30671331)

摘　　要：采用索氏提取法制取艾蒿(Artemisia argyi)乙醇粗提物,并设定5个不同的浓度,观察其对茶尺蠖3龄幼虫的生物活性。结果表明,各处理对茶尺蠖3龄幼虫具有较强的触杀、胃毒活性和一定的拒食及抑制生长发育的作用,提取物浓度越高,效果越明显。当提取物在100mg/mL浓度下,处理72h后,触杀和胃毒活性中的校正死亡率分别高达66.14%、78.16%;此浓度下,12h和24h的拒食率分别达到了56.77%和46.01%;24h和48h的生长抑制率分别为54.83%和68.32%。数据显示,艾蒿乙醇粗提物对茶尺蠖具有较好的毒杀作用,有开发成植物源杀虫剂的潜力,符合当前无公害茶叶生产之急需。Preparation of the Artemisia argyi by Soxhlet extraction and set the alcohol extracts of 5 different concentrations,observation of the Artemisia argyi extracts were bio-assayed against Ectropis obliqua larva.Results indicated that the extracts had many functional patterns against the larvae of Ectropis obliqua as anti-feeding,contacting toxicity,stomach poisoning and inhibiting growth activities.The higher concentration,the more obviously the effect on the pest was.In the test of contacting toxicity and stomach poisoning,the corrected mortality of 3 rd instar larvae of Ectropis obliqua reached 66.14% and 78.16% treated by contacting and poisoning at 72 h,respectively.For the anti-feeding,the results of the antifeedant effect showed that concentration of 100 mg/mL had good effect for the larva,the antifeedant effect were 56.77% and 46.01% after 12 h and 24 h,respectively.The extracts also had significant inhibition to growth and development of larvae the inhibition rate at 24 h and 48 h after treatment were 54.83% and 68.32% at concentration of 100 mg/mL.The date showed that Artemisia argyi extracts had good tea geometrid cytotoxicity,had developed into the potential of botanic insecticide,accorded with current of non-polluted tea production needs.

关 键 词：艾蒿 茶尺蠖 生物活性 胃毒 触杀 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]