纳米银溶胶的体外细胞毒性评价及其机制研究  

In vitro cytotoxicity and mechanism of silver colloid nanoparticles on mammalian cells

作　　者：杨桂利[1] 李俊杰[1] 林秋霞[1] 邱丽媛[1] 周瑾[1] 郝彤[1] 段翠密[1] 王常勇[1] 

机构地区：[1]中国人民解放军军事医学科学院基础医学研究所前沿交叉学科研究室,北京100850

出　　处：《国际生物医学工程杂志》2011年第5期279-283,I0003,共6页International Journal of Biomedical Engineering

摘　　要：目的对纳米银溶胶的体外细胞毒性进行评价，初步探讨纳米银对细胞的毒性作用机制。方法利用化学还原法制备纳米银溶胶，通过紫外一可见分光光度计和透射电镜对其物理特性进行检测；以小鼠纤维肉瘤细胞（L929）为研究对象，通过细胞形态观察、LIVE／DEAD染色分析和MTT检测来评价纳米银对细胞的毒性作用；在电子显微镜下观察纳米银在细胞中的分布，探讨纳米银对细胞的毒性作用机制。结果当纳米银质量浓度大于10mg／L时会对L929细胞产生明显的细胞毒性，细胞形态发生明显变化，细胞增殖受到抑制。纳米银可以通过胞吞作用进入细胞，与细胞内的多种细胞器相互作用而造成细胞损伤。结论纳米银会对哺乳动物细胞的形态和存活产生影响且具有浓度依赖性，因此在纳米银的应用过程中要考虑其毒副作用。Objective To evaluate the in vitro cytotoxicity of silver nanoparticles and investigate the toxicity mechanism of Nano Silver on cells. Methods Silver colloid was prepared by a modified chemical reduction method, and its physical properties were tested by ultraviolet-visible （UV-Vis） spectrometry and transmission electron microscopy （TEM）. The in vitro cytotoxicity of the silver nanoparticles was investigated using mouse fibrosarcoma cells （L929）. The toxicity was evaluated by the changes of cell morphology, LIVE/DEAD staining and 3-（4, 5-dimethyhhiazol-2-yl）-2, 5-diphenyl-tetrazolium bromide （MTT） assay. In order to study the biodistribution of Nano Silver in the cells, TEM analyses of the L929 cells treated with 20.0 μg/mL of nanoparticles were performed. Results Silver nanoparticle doses of 10 μg/mL could cause obvious toxicity to L929 cells, including morphology changes and the suppression of proliferation. The nanoparticles entered into the cells through endocytosis, and interacted with organelles to induce cellular damage. Conclusions The survival and morphology of mammal cells were significantly affected by silver nanoparticles in a dose dependent manner, and the cytotoxicity should be taken into account in the application of silver nanoparticles.

关 键 词：纳米银 细胞毒性 体外 作用机制 

分 类 号：TB383.1[一般工业技术—材料科学与工程]