毛细管电泳法测定雷公藤饮片中雷公藤红素的含量  

Quantitative analysis of celestrol in Leigongteng Herb drug by HPCE

作　　者：张婷[1] 

机构地区：[1]鲁东大学生命科学学院,山东烟台264025

出　　处：《化学工程师》2011年第11期25-27,共3页Chemical Engineer

摘　　要：采用毛细管电泳法对中药雷公藤中的成分进行分离并测定其有效成分雷公藤红素的含量。运行缓冲液为0.05mol.L-1磷酸盐（pH8.0）-15%甲醇;运行电压30kV,检测波长210nm,雷公藤红素的浓度在0.04~0.50mg.mL-1范围内线性关系良好,平均回收率为98.94%,RSD为1.72%（n=5）,回归方程为Y=321.41C＋1.96,r=0.9990。实验结果表明,运用毛细管电泳法可将中药雷公藤的有效成分雷公藤红素与其它成分较好地分离,并准确测定其含量。A capillary electrophoresis method for the determination of celestrol in Leigongteng was established.Herb drug.0.05mol·L-1 Phosphate buffer（pH8.0）-15%（V·V-1） methanol was used as running buffer.The running voltage was 30kV and the detection wavelenglth was set at 210nm.The calibration curve showed good linearity over the range of 0.04~0.50mg·mL-1（r=0.9990）.The average recovery was 98.94% and the relative standard deviation was 1.72%（n=5）.This method is accurate,proper and reliable for the determination of celestrol in Leigongteng Herb drug.

关 键 词：毛细管电泳 雷公藤红素 

分 类 号：O657.8[理学—分析化学]