三叶草绿变病植原体的分子鉴定  

Molecular detection and identification of a phytoplasma associated with Clover phyllody

作　　者：李正男[1] 刘萍[1] 吴云锋[1] 

机构地区：[1]旱区作物逆境生物学国家重点实验室植保资源与病虫害治理教育部重点实验室西北农林科技大学植物保护学院,杨凌712100

出　　处：《植物病理学报》2011年第6期645-648,共4页Acta Phytopathologica Sinica

基　　金：国家自然科学基金(30970133);高等学校博士学科点基金(20100204110004);高等学校学科创新引智计划(B07049)

摘　　要：三叶草(Trifolium pratense Linn.)为车轴草属,蝶形花科多年生草本植物,原产亚洲南部和欧洲东南部,是一种世界性分布与栽培的优良牧草。因其花叶兼优、草姿美、绿期长而具有较高的观赏价值,近几年作为草坪用草被广泛种植。在自然条件下,三叶草很容易受到不同种植原体的侵染,国外已报道的侵染三叶草的植原体有:三叶草绿变植原体(Clover phyllody phytoplasma,CPh)Clover phyllody,suspected to be infected by phytoplasma,was observed in Yangling,Shaanxi Province.Using phytoplasma universal 16S rRNA primer pairs R16mF2/R16mR1 and R16F2n/R16R2,the 1.2 kb product of expected size was amplified from clover exhibiting symptoms of phyllody.GenBank BLASTn analysis revealed that the phytoplasma shared highest similarity with members of 16SrV-B subgroup.Phylogenetic tree analysis indicated that the clover phytoplasma clustered together with the phytoplasmas of 16SrV-B subgroup.To our knowledge,this is the first report of phytoplasmas belonged to Elm Yellows group infecting clover in China.

关 键 词：三叶草 绿变现象 植原体 分子鉴定 

分 类 号：S432[农业科学—植物病理学]