逆转录环介导等温扩增技术检测花生条纹病毒  

Detection of Peanut stripe virus by the reverse transcription loop-mediated isothermal amplification method

作　　者：谢宏峰[1] 迟玉成[1] 许曼琳[1] 樊堂群[1] 张建建[1] 吴菊香[1] 许婷婷[1] 

机构地区：[1]山东省花生研究所,青岛266100

出　　处：《植物病理学报》2011年第6期649-653,共5页Acta Phytopathologica Sinica

基　　金：山东省优秀中青年科学家科研奖励基金(BS2009NY040)

摘　　要：花生条纹病毒病(Peanut stripe virus,PStV)是我国花生上流行最广[1]、田间发病率最高的一种花生病毒病,严重影响花生的产量和品质。由于PStV存在不同症状类型株系,在中国占优势的轻斑驳株系,引起花生症状较轻,并且与其他花生病毒病症状类似,不易分辨,目前主要利用PCR方法对PStV进行检测[2],但该方法检测时间长,并且需要专业仪器,较难普及。环介导等温扩增(Loop-mediated isothermal amplification,LAMP)A simple and sensitive Reverse Transcription Loop-mediated Isothermal Amplification（RT-LAMP） method was established to detect Peanut Stripe Virus（PStV）.A set of four specific primers were designed based on PStV coat protein gene for RT-LAMP assay.Amplification products were detected by SYBR green I staining and agarose gel electrophoresis.The specificity and sensitivity of the RT-LAMP assay were tested.The results showed that the RT-LAMP assay was rapid,specific and sensitive.The RT-LAMP reaction could be finished within 1 hour,and it was 10 times more sensitive than RT-PCR.The RT-LAMP assay reported here will become a useful method for rapid and reliable detection of PStV.

关 键 词：温扩增技术 花生 条纹病毒 逆转录环介导 

分 类 号：S432[农业科学—植物病理学]