固定化HRP/H_2O_2/ACAC酶促体系引发下降解淀粉接枝丙烯酰胺共聚物的合成与表征  

Synthesis and characterization of graft copolymer of degraded starch and acrylamide initiated by immobilized HRP/H_2O_2/ACAC catalyzed system

作　　者：闫小亮[1] 吕生华[1] 侯明明[1] 弓瑞[1] 

机构地区：[1]陕西科技大学资源与环境学院,陕西西安710021

出　　处：《化工进展》2011年第12期2708-2713,共6页Chemical Industry and Engineering Progress

基　　金：国家自然科学基金(20876091);陕西省自然科学基金(SJ08B06);陕西科技大学研究生创新基金项目

摘　　要：以壳聚糖为载体、戊二醛为交联剂制备固定化辣根过氧化物酶(HRP),采用固定化HRP/H2O2/乙酰丙酮(ACAC)酶促体系制备降解淀粉-丙烯酰胺接枝共聚物(St-g-PAM),通过红外光谱、紫外光谱、核磁共振和电镜扫描等手段对接枝共聚产物进行了结构分析。结果表明,丙烯酰胺成功接枝在降解淀粉上。共聚物用做皮革复鞣剂进行了应用实验,应用结果表明复鞣后的革柔软、纤维分散好和选择填充性强。Horseradish peroxidase（HRP）was immobilized on the surface of chitosan via glutaraldehyde crosslinking.Then a graft copolymer of degraded starch and acrylamide was synthesized by the initiation of enzyme catalysis system composed of chitosan-immobilized HRP/H2O2/acetylacetone（ACAC）.The structure of the copolymer was analyzed by FTIR,UV,NMR and SEM.The results indicated that polyacrylamide（PAM）was successfully grafted onto the degraded starch.Then,the copolymer was applied as a retanning agent.The applied results showed that the retanned leather had the merits of softness,good dispersion of fiber and strong selecting filling properties.

关 键 词：固定化辣根过氧化物酶 酶促聚合 接枝共聚 鞣剂 

分 类 号：TQ316.342[化学工程—高聚物工业]