辽阳地区稻水象甲对六种杀虫剂的敏感性及解毒酶活性测定  

Susceptibility to insecticide and detoxifying enzymes activity of rice water weevil,Lissorhoptrus oryzophilus in Liaoyang

作　　者：苏欣[1,2] 于娜 梁沛[1] 高希武[1] 

机构地区：[1]中国农业大学昆虫学系,北京100193 [2]辽阳市植保站,辽宁辽阳111000 [3]辽阳市农产品安全质量检验检测中心,辽宁辽阳111000

出　　处：《农药学学报》2011年第6期581-585,共5页Chinese Journal of Pesticide Science

基　　金：国家重点基础研究发展计划(973计划)课题(2009CB119200)

摘　　要：分别于2010和2011年采用点滴法测定了辽宁省辽阳地区张台子、柳壕和大石桥3个稻水象甲Lissorhoptrus oryzophilus Kuschel田间种群对吡虫啉等6种杀虫剂的敏感性。发现不同种群对同一药剂的敏感性相差可达4.2～49.4倍;2011年大石桥种群对阿维菌素等3种药剂的敏感性较2010年有所下降。通过室内毒力测定筛选出了5种可有效防治稻水象甲的药剂组合:阿维菌素和三唑磷、氯虫苯甲酰胺和三唑磷分别按质量比1∶1和1∶3组合,多杀菌素和辛硫磷按质量比1∶3组合。比较了3个种群稻水象甲成虫羧酸酯酶(CarE)、乙酰胆碱酯酶(AChE)和谷胱甘肽S-转移酶(GSTs)的活性,初步探讨了其敏感性不同的可能机制。研究结果可为该地区稻水象甲的有效控制提供参考。Three field populations of rice water weevil,Lissorhoptrus oryzophilus Kuschel,were collected in Liaoyang,Liaoning province during 2010-2011 and their susceptibility to six insecticides were investigated.The LD50s of the same insecticide to different populations varied from 4.2-to 49.4-fold.The susceptibility of Dashiqiao population to abamectin,triazophos and spinosad decreased slightly in 2011 compared to that in 2010.To control this beetle effectively,five insecticide mixtures that showed significant synergism were screened out through indoor bioassays,and the mixtures are abamectin mixed with triazophos at 1∶1 and 1∶3（m/m）,chlorantraniliprole mixed with triazophos at 1∶1 and 1∶3（m/m）,and spinosad mixed with phoxim at 1∶3（m/m）.The specific activity of acetylcholinesterase,carboxylesterase and glutathione S-transferase were analyzed among the three field populations and possible reasons for the difference in susceptibility were discussed.The results of the present work would be helpful for controlling the rice water weevil in Liaoyang area.

关 键 词：稻水象甲 杀虫剂 敏感性 解毒酶 

分 类 号：S482.3[农业科学—农药学] S481.1[农业科学—植物保护]