正交试验优选独一味总黄酮的提取工艺  

Optimization of Extraction Technology of Total Flavonoids from Lamiophlomis Rotata by Orthogonal Experiment

作　　者：王超[1] 王永[1] 宋剑[1] 李云鹏[1] 秦拢[1] 王猛[1] 安军永[1] 

机构地区：[1]石家庄以岭药业股份有限公司,河北石家庄050035

出　　处：《中国药业》2011年第22期57-59,共3页China Pharmaceuticals

摘　　要：目的建立独一味黄酮类成分的提取工艺。方法采用L9(34)正交设计法,以独一味总黄酮含量、山栀苷甲酯和8-O-乙酰山栀苷甲酯含量以及出膏率为检测指标进行试验。结果优选的提取工艺为,70%乙醇,提取3次、每次1.5 h,溶剂用量10倍。结论优选的独一味总黄酮类成分提取工艺简便、省时、节约成本,适合工业化生产。Objective To establish the extraction technology of total flavonoids from Lamiophlomis Rotata.Methods The L9（34） orthogonal design was adopted to perform the experiment with the content of total flavonoids,shanzhiside methyl ester and 8-O-acetyl shanzhiside methyl ester and the extraction rate as the detection indicators.Results The optimal extraction technology was as follows：70% ethanol,extracting for three times,each time for 1.5 h and 10 times the amount of solvent.Conclusion The optimized extraction technology of total flavonoids from Lamiophlomis Rotata is simple,time-saving,cost savings and suitable for industrial production.

关 键 词：独一味 正交试验 提取工艺 总黄酮 

分 类 号：TQ461[化学工程—制药化工] R282.71[医药卫生—中药学]