年龄相关性黄斑变性中医证型与CFH基因Y402H、CFB基因R32Q多态性的相关性研究  

Research on the Correlation Between TCM Syndrome Patterns of Age-related Macular Degeneration and CFH Gene Y402H and CFB Gene R32Q Polymorphisms

作　　者：仲路[1] 姚静[2] 高峰[1] 周欣[1] 赵玥[2] 徐新荣[1] 

机构地区：[1]南京中医药大学附属医院,江苏南京210029 [2]南京医科大学附属眼科医院,江苏南京210029

出　　处：《南京中医药大学学报》2011年第6期516-518,共3页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省"六大人才高峰"第五批资助项目;江苏省中医药领军人才培养对象资助项目

摘　　要：目的探讨补体因子H(CFH)及补体因子B(CFB)基因变异与年龄相关性黄斑变性(AMD)中医证型的相关性。方法门诊确诊AMD患者125例,按脾虚湿困、痰瘀互结及肝肾亏虚3个证型分成3组。采集患者的外周血,提取DNA,PCR扩增后测序,检测CFH与CBF单核昔酸多态性,分析所得到的基因变异结果与AMD中医证型的相关性。结果①AMD 3个证型组中,干性与湿性病例数分布有显著性差异(χ2=33.524,P<0.01),脾虚湿困证以干性为主,肝肾亏虚证以湿性为主,痰瘀互结证则干性、湿性比大致相当。②3组CFH基因Y402H的基因型频率比较差异有显著性(χ2=10.036,P<0.05);等位基因T/C频率分布比较差异有显著性(χ2=9.501,P<0.01),其中肝肾亏虚证等位基因C型比例最高(23.44%)。③3组CFB基因R32Q的基因型频率比较差异有显著性(χ2=6.171,P<0.05),等位基因G/A频率分布比较差异有显著性(χ2=10.455,P<0.01),其中脾虚湿困证等位基因A型比例最高(16.67%)。结论 AMD脾虚湿困证多数是AMD早期病例,肝肾亏虚证多数是AMD晚期病例;肝肾亏虚证与CFH基因Y402H中TC/CC基因型及等位基因C相关,脾虚湿困证与CFB基因R32Q的GA基因型及等位基因A相关。OBJECTIVE To investigate the correlation between gene variations of complementary factor H（CFH） and B（CFB） and TCM syndrome patterns of age-related macular degeneration（AMD）.METHODS 125 AMD patients confirmed in the outpatient were divided into 3 groups according to 3 syndrome patterns,namely spleen deficiency with dampness retention,mutual accumulation of phlegm and dampness and consumption of liver and kidney.Peripheral blood of patients was collected to extract DNA.After PCR amplification,its sequence was measured to detect single nucleotide polymorphisms（SNP） of CFH and CBF,and the correlation between acquired gene variations and TCM syndrome patterns of AMD was analyzed.RESULTS ①Among 3 syndrome patterns of AMD,there were significant differences with regard to the distribution of the number of dry and damp cases（χ2=33.524,P0.01）,the syndrome of spleen deficiency with dampness retention focusing on dryness,the syndrome of consumption of liver and kidney on dampness,the proportion between dryness and dampness of the syndrome of mutual accumulation of phlegm and dampness being approximately equal;②Compared with CFH gene Y402H genotype frequency in 3 groups,conspicuous differences were presented（χ2=10.036,P0.05）;compared with allele T/C frequency distribution,significant differences were shown（χ2=9.501,P0.01）,in which allele type C of the syndrome of consumption of liver and kidney manifested the highest proportion（23.44%）;③Compared with CFB gene R32 genotype frequency in 3 groups,noticeable differences were presented（χ2=6.171,P0.05）;compared with allele G/A frequency distribution,significant differences were shown（χ2=10.455,P0.01）,in which allele type A of the syndrome of spleen deficiency with dampness retention showed the highest proportion（16.67%）.CONCLUSION The syndrome of spleen deficiency with dampness retention usually occurs at the early stage of AMD while the syndrome of consumption of liver and kidney mostly at the late stage;the former is related

关 键 词：年龄相关性黄斑变性 中医证型 补体因子H 补体因子B 单核苷多态性 

分 类 号：R276.7[医药卫生—中医五官科学]