近红外光谱法测定天麻中天麻素的含量  

Determination of Gastrodine Content in Rhizoma Gastrodiae by Near-infrared Spectroscopy

作　　者：杜伟锋[1] 徐珊珊[1] 王胜波[1] 丛晓东[1] 张云[1] 

机构地区：[1]浙江中医药大学中药炮制技术研究中心,浙江杭州311401

出　　处：《南京中医药大学学报》2011年第6期568-569,共2页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：浙江省卫生厅省部共建项目(WKJ2010-2-019);浙江中医药大学校级科研基金(17110037)

摘　　要：目的采用近红外光谱法对不同产地的天麻中天麻素含量进行快速测定。方法以HPLC分析值作为参照,采用傅里叶变换近红外漫反射光谱技术采集天麻的近红外光谱,结合偏最小二乘法(PLS)建立天麻素含量的快速测定方法。结果天麻素校正模型的相关系数(R)、校正均方差(RMSEC)、内部验证均方差(RMSEP)分别为0.990 6、0.043 2、0.032 4。经外部验证,预测值与真实值的相关系数达0.996 6。结论该方法准确、快速、简便,可实现大批量样品的快速分析。OBJECTIVE A near-infrared quantitative method was developed for rapid determination of gastrodine in Rhizoma Gastrodiae.METHODS Near-infrared diffuse reflectance spectroscopy technology was adopted to collect NIR spectra of Rhizoma Gastrodiae with HPLC analysis values as reference,the rapid determination method of gastrodine was established with partial least squares（PLS）.RESULTS The correlation coefficients,the root-mean-square error of calibration（RMSEC） and the root-mean-square error of validation（RMSEP） of the calibration model for gastrodine were 0.990 6,0.043 2 and 0.032 4,respectively;the correlation coefficient of the true value and prediction value from external validation was 0.996 6.CONCLUSION The method is accurate,fast and simple,which can be applied for the rapid determination of large quantities of numbers of samples.

关 键 词：近红外光谱法 天麻 天麻素 

分 类 号：R284.1[医药卫生—中药学]