RP-HPLC测定水蛭中3种蝶啶类活性成分的含量  

Content Determination of Three Pteridine Active Ingredients in Leech by RP-HPLC

作　　者：郑云枫[1] 程建明[1] 彭国平[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210046

出　　处：《南京中医药大学学报》2011年第6期573-574,共2页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省自然科学基金(SBK201123752)

摘　　要：目的建立RP-HPLC测定水蛭药材中3种蝶啶类活性成分含量的方法。方法采用Kromsail C18色谱柱,以甲醇:0.05%三氟乙酸水(20∶80)为流动相,检测波长为354nm,流速为1.0mL/min,柱温30℃。结果在以上色谱条件下3种成分可以完全分离。水蛭甲素:Y=12.181 4 X+0.075 3,r=0.999 6;(36.0～0.90)μg/mL,水蛭乙素:Y=12.024X-2.124 5,r=0.999 5;(40.4～1.01)μg/mL,水蛭丙素:Y=12.814 5 X-3.686 1,r=0.999 8;(42.8～1.07)μg/mL。结论该方法简单、快速,专属性好,可用于水蛭药材的质量控制方法之一。OBJECTIVE To establish RP-HPLC determination of the content of three pteridine active ingredients in leech.METHODS Kromsail C18 chromatographic column was used with methanol to 0.05% trifluoroacetic acid（20∶80） as mobile phase,detecting wave length of 354 nm,flow speed of 1.0 mL/min and column temperature of 30 ℃.RESULTS Three active ingredients were separated on the conditions mentioned above.Hirudin A： Y=12.181 4X＋0.075 3,r=0.999 6;（36.0~0.90）μg/mL.Hirudin B：Y=12.024 X-2.124 5,r=0.999 5;（40.4~1.01）μg/mL.Hirudin C： Y=12.814 5X-3.686 1,r=0.999 8;（42.8~1.07）μg/mL.CONCLUSION The method proves to be easy and fast with good specificity,which can be used to control quality of leeches.

关 键 词：水蛭 蝶啶类 活性成分 HPLC 

分 类 号：R284.1[医药卫生—中药学]