薄层分离-分光光度法测定夹竹桃枝中三萜类化合物的质量分数  

Quantitative analysis of triterpenoids from the bark of Nerium oleander L by thin layer chromatography-spectrophotometry

作　　者：孟繁旭[1] 白丽明[1] 马玲[1] 安东政义[2] 

机构地区：[1]齐齐哈尔大学化学与化学工程学院,黑龙江齐齐哈尔161006 [2]日本新澙大学

出　　处：《高师理科学刊》2011年第6期59-61,共3页Journal of Science of Teachers＇College and University

基　　金：教育部留学回国人员科研启动基金(2009-36-1341);黑龙江省留学归国科学基金(LC201008)

摘　　要：为测定夹竹桃中总三萜的质量分数,以齐墩果酸为对照品,5%的香草醛-冰醋酸溶液和高氯酸为显色剂,采用薄层色谱分离-分光光度法在550 nm波长处测定样品吸光度.用正交试验法优化了显色反应条件,适宜的展开剂组成为氯仿∶丙酮=10∶3,对照品在10～200μL/mL范围呈良好线性关系,其回归方程为Y=0.0070C+0.0317,r=0.998 6.夹竹桃中总三萜的质量分数为1.01%.该方法适合夹竹桃中总三萜的质量分数测定,操作简单,灵敏度高且比较准确.Objective to determination the triterpenoids content in the bark of Nerium oleander L. Under the experimental conditions including oleanolie acid as the reference. 5% vanillin-acetie acid and perehloric acid as color developers, the content of triterp-enoids in Nerium oleander L was determined by the thin laver ehromatographv-spectrophotometric method at 550 nm. The condition was optimized by orthogonal design. The results showed that chloroform： acetone=10 ： 3 as developer, the calibration curve was linear（ r=0. 9986 ）in the range of 20-200 tx L/mL, and the regression equation was Y= 0. 007 0C ＋ 0. 031 7. The content of triterpenoids in the bark of Nerium oleander L was about 1.01%. The spectrophotometry is suitable for the determination of total triterpenoids from Nerium oleander L. This method is convenient, stable and reproducible.

关 键 词：夹竹桃 三萜类化合物 分光光度法 薄层色谱 

分 类 号：R284.2[医药卫生—中药学]