SPE-HPLC-ELSD法测定补虚颗粒中黄芪甲苷的含量  

Determination of Astragaloside Ⅳ in Buxue Granules by SPE-HPLC-ELSD

作　　者：张滔[1] 方宝霞[1] 于琳[1] 时晓亚[1] 

机构地区：[1]湖北医药学院附属东风医院药学部,湖北十堰442008

出　　处：《中医药导报》2011年第11期70-71,共2页Guiding Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:建立补虚颗粒中黄芪甲苷含量的测定方法。方法:采用固相萃取-高效液相色谱-蒸发光散射检测(SPE-HPLC-ELSD)法,以Kromasil C18为色谱柱分离,乙腈∶水(35∶65)为流动相;流速:0.8 mL.min-1;柱温:30℃。结果:黄芪甲苷进样量在1.03-20.6μg范围内具有良好的线性关系(r=0.9995);平均回收率为98.74%,RSD%为0.89%(n=6)。结论:本方法简便、快速,分离度好,结果准确可靠,可作为补虚颗粒的质量控制方法。Objeetive：To establish a HPLC method for determination the content of Astragaloside Ⅳ in Buxue Granules.Method：SPE-HPLC-ELSD was used in the quantitative analysis with Kromasil C18 chromatography column（4.6 mm×250 mm,5 μm） and acetonitrile-water（35：65） as a mobile phase.The flow rate of mobile phase was 0.8 mL·min-1.Result：The calibration curve for astragaloside Ⅳ was in good linearity within the range of 1.03-20.6 μg（r=0.9995）,the average recovery was 98.74%,RSD was 0.89%（n=6）.Conclusion：The method with good separation is convenient,rapid,accurate and reliable,and can be regarded as the quality control method of Buxue Granules.

关 键 词：补虚颗粒 黄芪甲苷 HPLC 固相萃取 

分 类 号：R284.1[医药卫生—中药学]