空间诱变辣椒AFLP反应体系的优化  

Optimization of AFLP System for Hot Pepper Induced by Space

作　　者：周春阳[1] 郭亚华[2] 谢立波[2] 王雪[2] 高永利[2] 周宇[2] 黄凤兰[3] 彭木[3] 

机构地区：[1]东北农业大学园艺学院,黑龙江哈尔滨150030 [2]黑龙江省农业科学院园艺分院,黑龙江哈尔滨150069 [3]内蒙古民族大学生命科学学院

出　　处：《北方园艺》2011年第21期103-105,共3页Northern Horticulture

基　　金：航天育种工程资助项目(2006HT20012);国家大宗蔬菜产业技术体系资助项目(CARS-25-G-11);黑龙江省自然科学基金重点资助项目(ZD200818-01);黑龙江省自然科学基金资助项目(C200943)

摘　　要：为了建立适合空间诱变辣椒的AFLP反应体系,以3～4叶期的辣椒叶片为试材,对体系中的DNA用量、酶切与连接、预扩增及产物的稀释倍数等关键因素进行了优化,确立了适合空间诱变辣椒的AFLP反应体系。结果表明:采用该体系对空间诱变辣椒进行AFLP分析,能够得到清晰稳定的分子标记图谱。In order to establish a suitable AFLP reaction system of pepper induced by space,taking 3~4 leaf stage of pepper leaves as test materials,several significant influencing factors,including the dosage of template DNA,enzyme digestion and ligation,pre-amplification and the dilute of pre-amplification products were optimized,and a suitable AFLP reaction system for hot pepper induced by space was constructed.The results showed that by this optimized system,a clear and credible AFLP fingerprint could be obtained.

关 键 词：空间诱变 辣椒 AFLP反应体系 优化 

分 类 号：S641.303.6[农业科学—蔬菜学]