甘蓝胞质雄性不育系育性相关线粒体DNA片断的克隆及序列分析  

Cloning and Sequencing of a Mitochondrial DNA Fragment Related to Cytoplasmic Male Sterile Line of Cabbage

作　　者：许忠民[1] 张恩慧[1] 巩振辉[1] 程永安[1] 马青山[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《北方园艺》2011年第21期106-110,共5页Northern Horticulture

基　　金：国家"十一五"科技支撑计划资助项目(2008BADB1B02);陕西省农业科技攻关计划资助项目(2009K01-24);西安市农业科技攻关计划资助项目(NC09041(1));西北农林科技大学唐仲英植物育种专项资助项目

摘　　要：以甘蓝结球期叶球内叶为试材,利用PCR方法在甘蓝新型胞质雄性不育材料CMS451的线粒体基因组中扩增出与细胞质雄性不育相关的片断,并克隆测序。结果表明:该片断和NCBI发布的Ogura胞质不育系特异片段高度同源,说明CMS451的不育机理与Ogu-CMS相似,也反映了CMS基因的相对保守性。Taking ball leaves of cabbage in the period of ball as test materials,the special sequence related to CMS was amplified in the new e cabbage CMS451 by PCR,and its homology was highly with Ogu-CMS published in NCBI.The results showed that CMS451 had the similar sterility with Ogu-CMS,but the gene related to CMS was relative conservative.

关 键 词：甘蓝 CMS451 细胞质雄性不育(CMS) 线粒体DNA(mtDNA) 

分 类 号：S635[农业科学—蔬菜学]