原子力显微镜扫描成像DNA分子  

Imaging of DNA molecules with atomic force microscopy

作　　者：冉诗勇[1] 王艳伟[1] 杨光参[1] 

机构地区：[1]温州大学物理与电子信息工程学院,浙江温州325035

出　　处：《物理实验》2011年第11期1-4,12,共5页Physics Experimentation

基　　金：浙江省自然科学基金资助项目(No.Y4110357);温州大学研究生教改项目(No.YJG200909)

摘　　要：采用Mg2+处理DNA、APTES或戊二醛修饰云母表面、DNA拉直方法制备了λ-DNA及DNA-组蛋白复合物样品.室温下原子力显微镜以轻敲模式在空气中扫描样品成像.实验结果表明:AFM扫描成像的效果与样品的制备方法有关,同时也受操作因素影响.DNA molecules and DNA-histone complexes were deposited on mica using four methods： adding Mg2＋ to DNA solution,modifying mica surface by 3-aminopropylrtiethoxysilane or glutaraldehyde,and straightening DNA.The scanning images of the four samples were obtained at room temperature with atomic force microscopy working in taping mode.The experimental results indicated that the quality of the image was affected by the deposition method and the operation procedure.

关 键 词：AFM DNA 成像 

分 类 号：Q6-33[生物学—生物物理学]