花椰菜菌核病抗性鉴定方法比较及抗病种质资源筛选  

Comparison of identification methods for resistance to Sclerotinia sclerotiorum and screening of resistant materials in cauliflower

作　　者：孙溶溶[1] 彭真[1] 程琳[1] 邵泰良 卢钢[1] 

机构地区：[1]浙江大学农业与生物技术学院蔬菜研究所,浙江杭州310058 [2]浙江神良种业有限公司,浙江温州325011

出　　处：《浙江大学学报（农业与生命科学版）》2011年第6期596-602,共7页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：浙江省科技厅重大科技专项国际科技合作资助项目(2008C14066)

摘　　要：采用已报道的3种十字花科作物菌核病抗病性鉴定方法,在同一条件下,对5个已知抗性的花椰菜品种进行鉴定比较.结果表明:结球期琼脂块叶腋接种法、营养生长期琼脂块活体叶片法与大田接种鉴定的相关系数分别达到0.885 9和0.926 6,可有效反映抗、感品种的差异,可用于花椰菜菌核病抗性的快速鉴定;但营养生长期琼脂块离体叶片法用于抗性鉴定的效果不好;根据抗性鉴定方法的比较结果,选用结球期琼脂块叶腋接种法,以农乐45天为对照,对21份花椰菜材料进行抗性鉴定,筛选获得了神良金花和长胜65天2份高抗材料.Three identification methods for the resistance to Sclerotinia sclerotiorum previously-reported in Brassicaceae crops were compared using 5 cauliflower varieties under the same condition to develop the effective screening technique in cauliflower.The results showed that the correlation between inoculating mycelium agar disk to leaf axil at seedling stage,or inoculating mycelium agar disk to live leaf at heading stage and field inoculation were highly significant（r=0.8859 and 0.9266,respectively）,so these two methods could well identify resistance and susceptibility of cauliflower varieties.The method of inoculating mycelium agar disk to in vitro seedling leaf was not suitable for resistance identification of cauliflower.To screen resistant sources of cauliflower against S.sclerotiorum,21 breeding materials were tested with the method of inoculating mycelium agar disk to leaf axil at heading stage,using cauliflower variety ＇nongle 45 days＇ as control.The results showed that there were two high resistant materials,＇shenliang golden flower＇ and ＇changsheng 65 days＇.

关 键 词：花椰菜 菌核病 抗病性 鉴定方法 种质资源 

分 类 号：Q93-332[生物学—微生物学] S635.3[农业科学—蔬菜学]