猪Mx1基因cDNA的克隆及序列分析  

Cloning and Sequence Analysis of Porcine Mx1 Gene cDNA

作　　者：李想[1] 沈学文[2] 李文贵[1] 毕峻龙[1] 杨贵树[1] 尹革芬[1] 

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]云南省红河州动物疫病预防控制中心,云南红河661100

出　　处：《云南农业大学学报（自然科学版）》2011年第6期771-775,789,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然科学基金(30860205)

摘　　要：为获得云南本地猪Mx1基因cDNA序列,根据GenBank中猪Mx1基因的参考序列,设计合成3对引物。以云南本地猪外周淋巴细胞为样本,采用RT-PCR方法进行分段扩增,对扩增片段进行克隆,测序分析及序列拼接。结果表明,扩增的云南本地猪Mx1基因cDNA全长2 546 bp,开放阅读框1 992 bp,编码663个氨基酸,与GenBank中他猪种Mx1基因序列对比,核苷酸序列的同源性为99.4%～99.8%,氨基酸序列的同源性为98.8%～99.5%。成功获得云南本地猪Mx1基因的cDNA序列,为研究云南本地猪Mx1蛋白的抗病毒活性及作用机制奠定了基础。In order to obtain the cDNA sequence of Mxl gene of Yunnan local pig, primers were designed according to the sequences of porcine Mxl gene in GenBank. Fragments were amplified using RT-PCR from peripheral lymphocytes of Yunnan local pigs, and the fragment sequences were analyzed and spliced. The results demonstrated that the cDNA length of Mxl gene amplified from Yunnan local pigs was 2 546 bp, the open reading frame was 1 992 bp with encoding 663 amino acids. Compared with other Mxl gene sequences in GenBank, the homologies of nucleotide sequences were from 99.4% to 99. 8%, those of amino acid sequences were from 98.8% to 99. 5%. The cDNA sequence of Mxl gene in Yunnan local pigs was successfully obtained, which may facilitate the study on the antiviral ac- tivity and mechanism of Mxl protein in Yunnan local pigs.

关 键 词：Mx1基因 克隆 序列分析 猪 

分 类 号：S828[农业科学—畜牧学]