SBHL对小鼠免疫功能影响的实验研究  

The experimental investigation of Sdosodine Hydrochlorida on immune function in mice

作　　者：崔焕波[1] 艾金霞[2] 

机构地区：[1]吉林省公安边防总队医院检验科,吉林长春130051 [2]北华大学医学院免疫教研室,吉林吉林132013

出　　处：《中国实验诊断学》2011年第11期1804-1806,共3页Chinese Journal of Laboratory Diagnosis

基　　金：吉林市卓依康纳中药研发基金(ZY040301)

摘　　要：目的探讨澳洲茄胺盐酸盐(SBHL)对环磷酰胺(CTX)处理小鼠免疫功能的影响。方法 Balb/c小鼠用环磷酰胺造成免疫抑制模型,同时尾静脉注射SBHL7d,观察小鼠免疫器官重量,脾细胞特异性抗体的产生、血清凝集素滴度、溶血素CH50值、ConA刺激的小鼠脾淋巴细胞增殖和小鼠腹腔巨噬细胞吞噬功能及血清中肿瘤坏死因子α含量的变化。结果 SBHL(40 mg/kg)尾静脉注射7 d,小鼠胸腺、脾脏重量增加。ConA刺激的小鼠脾淋巴细胞增殖明显。小鼠腹腔巨噬细胞吞噬功能显著增强。给药组小鼠血凝素滴度、溶血素CH50值、TNF-α也高于对照组。结论 SBHL能明显的改善环磷酰胺抑制的小鼠免疫功能,这可能是澳洲茄胺盐酸盐抗肿瘤的另一作用机理。Objective To explore the improving immune function of the Sdosodine Hydrochlorida（SBHL）in mice treated with cyclophosphamide（CTX）.Methods Balb/c mice treated with CTX were administrated with SBHL（40 mg/kg） for 7 d.The weights of spleens and thymus of the mice,specific antibody formation of splenocytes,serum agglutinin titer and hemolysin CH50 in mice immunized with SRBC were assayed.The proliferation of mouse splenic lymphocytes actived with ConA were measured.The peritoneal macrophage phagocytosis in mice were analysized.Results After treatment with SBHL,the thymus of mice,the proliferation of mouse splenic lymphocytes activated with ConA were increased markedly,indose-dependent manner.The peritoneal macrophage phagocytosis in the mice were also improved.Only at dose of 40mg/kg were the weights of spleens of mice,specific antibody formation of splenocytes,serum agglutinin titer,hemolysin CH50 in mice immunized with SRBC increased significantly.Conclusion SBHL had a remarkable improving effects on immune function inhibited by CTX in mice,which may be another important antitumor mechanism of SBHL.

关 键 词：免疫功能 澳洲茄胺盐酸盐 动物实验 

分 类 号：R285.5[医药卫生—中药学]