胚胎移植技术在扩繁德系西门塔尔牛核心群中的应用  

Application of Embryo Transfer on Reproduction of German Simmental Core

作　　者：毕江华 冯春涛 朱士恩[2,3] 余文莉 

机构地区：[1]石家庄天泉良种奶牛有限公司,石家庄050051 [2]中国肉牛产业技术体系遗传育种与繁殖功能研究室,北京100193 [3]中国农业大学动物科技学院,北京100193

出　　处：《中国农学通报》2011年第26期43-46,共4页Chinese Agricultural Science Bulletin

基　　金：现代农业产业技术体系建设专项资金资助(nycytx-38);"十二五"科技支撑课题(2011BAD19B02)

摘　　要：13头德系西门塔尔供体牛连续4次重复超排,生产胚胎654枚(头均50.31枚),可用胚胎335枚(头均25.77枚);4次冲胚的平均回收胚数,可用胚数差异均不显著(P>0.05)。移植受体牛278头次(鲜胚移植115头,冻胚移植118头,双半胚移植45头),妊娠率55.04%(153/278),共获得犊牛137头。其中,鲜双半胚和鲜胚移植妊娠率显著高于冻胚(64.44%,60.87%vs45.76%)(P<0.05)。45枚鲜胚二分后移植到45头受体牛,29头妊娠,其中11头产双胎,双犊率为37.93%(11/29),存活犊牛29头。13 German Simmental donor cows for 4 times repeated superovulation,654 embryos were obtained （the average number of embryos per cow was 50.31）,and the available embryo was 335 （the average number of embryos per cow was 25.77）.The results of donors were flushed repeatedly for 4 times showed the average recovery of embryos,transferable embryos had no significantly different （P0.05）.278 recipients were transplanted （115 use fresh embryo,118 use frozen embryo,45 use pairs of demi-embryos） followed by pregnancy rate of 55.04% （135/278）,total of 137 calves.Both pairs of demi-embryos and fresh embryos transfer pregnancy rate were significantly higher than that of frozen embryos （64.44%,60.87% vs 45.76%） （P 0.05）.45 fresh Pairs of demi-embryos were transferred to 45 recipients,29 were pregnant,among them,there were 11 identica1 twins with twin rate of 37.93% （11/29）,29 calves survived.

关 键 词：重复超排 分割双半胚 胚胎移植 双犊率 德系西门塔尔牛 

分 类 号：S823[农业科学—畜牧学]