玉米γ-生育酚甲基转移酶基因的克隆与分析  

Cloning and Sequence Analysis of γ-Tocopherol Methyltransferase (γ-TMT) cDNA from Maize(Zea mays)

作　　者：邹礼平[1] 

机构地区：[1]孝感学院生命科学技术学院,湖北孝感432000

出　　处：《安徽农业科学》2011年第33期20316-20317,20321,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]对玉米γ-生育酚甲基转移酶(γ-TMT)基因进行克隆和分析研究。[方法]通过RT-PCR扩增得到玉米γ-TMT基因的cDNA序列,同时对序列进行同源性分析、氨基酸序列比对和系统进化分析。[结果]玉米γ-TMT基因cDNA全长1 310 bp,包含一个长为1 059bp的完整开放读码框,推导的氨基酸序列与小麦和水稻等高等植物的γ-TMT基因同源性较高,序列一致率为69%～87%;与低等生物褐藻的序列同源性较低,一致率只有56%。系统进化分析表明不同来源的γ-TMT基因可聚为3类。[结论]为进一步研究γ-TMT基因的功能奠定了基础。[Objective] The aim was to clone and analyze the sequence of γ-tocopherol methyltranferase（γ-TMT） cDNA from maize.[Method] The cDNA of maize γ-TMT was cloned by RT-PCR and then its sequence homology analysis,sequence alignment of amino acids and phylogenetic analysis were done.[Result] The full-length cDNA sequence of maize γ-TMT was 1 310 bp,containing a complete open reading frame（ORF） of 1 059 bp.Homology analysis of the deduced amino acid showed 69%-87% identity with γ-TMT from plants and only 56% identity with γ-TMT from Ectocarpus siliculosus.The result of phylogenetic analysis indicated that various γ-TMT sequences were clustered into 3 groups.[Conclusion] It prepared the ground for the further research of γ-TMT function.

关 键 词：玉米 Γ-生育酚甲基转移酶 维生素E 生物合成 基因克隆 

分 类 号：S188.1[农业科学—农业基础科学]