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作 者:杨志兵[1] 杨佩佩[1] 贺斌[2] 傅丽容[1] 史海涛[1] 魏朝军[1] 陈美玲[1]
机构地区:[1]海南师范大学生命科学学院,海南海口571158 [2]浙江大学动物科学学院,浙江杭州310058
出 处:《安徽农业科学》2011年第33期20501-20505,20573,共6页Journal of Anhui Agricultural Sciences
基 金:海南省自然基金资助项目(310040);海南省教育厅资助项目(Hjkj2010-24);国际地区重大合作项目(30910103916)
摘 要:[目的]研究中华条颈龟(Mauremys sinensis)胚胎发育过程中外部形态的变化。[方法]在恒温(29±0.5)℃,相对湿度为85%~90%,基质湿度为5%~10%的孵化条件下,连续解剖观察了中华条颈龟胚胎发育的过程。对胚胎发育各期主要形态学特征进行了详细描述,并配以周详的图片比较各期的典型特征。[结果]中华条颈龟的孵化期为53.00±1.77 d,根据其胚胎的日龄、大小和形态特征的变化将整个的胚胎发育分成了23期,其中9~23期主要以四肢、背甲、腹甲、头部、尾部等的变化为分期依据,同时,对龟鳖类胚胎发育分期的标准进行了讨论。[结论]结果有利于龟鳖类胚胎发育分期的标准化。[Objective] The aim was to observe the morphological changes of embryonic development of M.sinensis.[Methods] Embryonic development process of M.sinensis were observed based on serial dissection,under the condition of constant temperature(29±0.5)℃,relative humidity 85%-90%,5%-10% of matrix humidity,and the major morphological characters of every stage were described in detail with amply photos respectively.[Results] The incubation period of M.sinensis were 53.00±1.77 d,according to the embryo daily age,size,and morphological characters,the period of embryonic development was divided into twenty-three stages,hereinto,stages 9 to 23 were affirmed mainly by the shape changes of limbs,carapace,plastron,head and tail;meanwhile,staging criteria of embryonic development of chelonia was discussed here.[Conclusion] The results were beneficial to the standardization of morphological development of M.sinensis.
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