白肋烟青枯菌的分离鉴定及药敏性研究  

Identification and Susceptibility Test of Ralstonia solanacearum Isolated from White Burley

作　　者：李洪[1] 严占勇[2] 杨水英[3] 吴卫玲[1] 王晗[1] 李振轮[1] 

机构地区：[1]西南大学资源环境学院,重庆400716 [2]四川省烟草公司达州市公司,四川达州635006 [3]西南大学植物保护学院,重庆400716

出　　处：《安徽农业科学》2011年第34期21082-21084,共3页Journal of Anhui Agricultural Sciences

基　　金：四川省烟草专卖局项目(200901009)

摘　　要：[目的]分离鉴定白肋烟青枯菌并测定该菌对6种杀菌剂的药敏性。[方法]采用半选择培养基(PCCG)和聚合酶链式反应(PCR)技术相结合的方法,对四川省达州市白肋烟茎秆中的青枯菌进行分离,并对其生化型进行了鉴定;通过药敏试验,研究了所得青枯病菌在实验室条件下对叶枯唑、乙蒜素、农用硫酸链霉素、石硫合剂、47%聚赖氨酸、99%曲酸6种杀菌剂的敏感性。[结果]共分离得到23株青枯菌,均为生化Ⅲ型。分离所得青枯菌株对乙蒜素、农用硫酸链霉素、叶枯唑较为敏感,其中乙蒜素对烟草青枯病菌有良好的防治效果,其EC50为0.086 ml/L。[结论]为白肋烟青枯病的防治提供了理论依据。[Objective] The paper was to isolate and identify Ralstonia solanacearum isolated from white burley,and determine its susceptibility to 6 fungicides.[Method] Using the combination method of semiselective medium（PCCG） and apolymerase chain reaction（PCR） technique,R.solanacearum in haulm of white burley from Dazhou City in Sichuan Province was isolated,and its biochemical type was identified.Through susceptibility test,the susceptibility of R.solanacearum to bismerthiazol,ethylicin,streptomycin,lime sulfur,47%Poly-L-lysine hydrochloride,99%kojic acid was studied in laboratory.[Result] A total of 23 strains of R.solanacearum were isolated,all belonging to biochemical type Ⅲ.R.solanacearum obtained in the test were more susceptible to ethylicin,streptomycin and bismerthiazol,and ethylicin had good control effect against R.solanacearum with EC50 of 0.086 ml/L.[Conclusion] The study provide theoretical basis for control of R.solanacearum in white burley.

关 键 词：白肋烟青枯菌 细菌分离 鉴定 PCCG 药敏性测试 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]