高压氧对老年痴呆模型小鼠抗痴呆作用的实验研究  

Effect of hyperbaric oxygen on the anti-senility of mice with Alzheimer disease

作　　者：周宏图[1] 周红[2] 严丽荣[3] 袁建国[1] 李绒[1] 张雪春[1] 周玉梅[1] 

机构地区：[1]江苏大学附属医院高压氧科,江苏省镇江212001 [2]江苏大学基础医学与医学技术学院 [3]江苏大学附属医院老年医学科,江苏省镇江212001

出　　处：《中华航海医学与高气压医学杂志》2011年第5期288-291,共4页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：江苏大学青年科研基金资助项目（JFY2006013）

摘　　要：目的探讨高压氧对老年痴呆小鼠（AD）的抗痴呆作用。方法将30只昆明种雄性小鼠按数字表法随机均分成3组：对照组（不作任何处理）、模型组（建成痴呆模型后不作任何处理）、高压氧组（建模后进行高压氧治疗），每组10只。后2组用D-半乳糖（120mg／kg）和亚硝酸钠（90mg／kg）联合制作老年痴呆小鼠模型，利用Morris水迷宫试验进行逃避潜伏期、跨越平台次数的检测；采用酶生化法和硫代巴比妥酸法进行血清超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH—Px）活性和丙二醛（MDA）含量检测；取小鼠海马CA1区脑组织行HE染色，观察神经细胞形态变化。结果与对照组比较，模型组逃避潜伏期[（76．13±25．28）S]明显延长（P〈0．01），跨越平台次数[（2．34-4-0．87）次]明显减少（P〈0．01），血清中SOD[（269．70±33．45）U／m1]、GSH—Px[（4．38±1．21）g/L]活性显著降低（P〈0．01），MDA[（6．57±2．04）μmol／L]含量显著升高（P〈0．01），海马CAl区神经细胞排列稀疏、紊乱，出现大量退行性神经元；模型组经高压氧治疗后，与治疗前比较逃避潜伏期明显缩短（P〈0．01）、跨越平台次数明显增多（P〈0．01），血清SOD、GSH—Px活性显著升高（P〈0．01），MDA含量显著降低（P〈0．01），治疗前后差异均有统计学意义（P〈0．01），而且海马CA1区正常神经细胞数量较治疗前明显增多、细胞排列紧密。结论高压氧治疗可以显著提高痴呆模型鼠的抗自由基损伤能力，具有抗痴呆功能，对AD具有一定的治疗作用。Objective To study the effect of hyperbaric oxygen on the anti-senility of mice with Alzheimer disease. Methods Thirty male KM mice were randomly divided into 3 groups： the control group, the model group and the HBO group. Models of dementia mice were successfully developed with D-galaetose （120 mg/kg） and sodium nitrite （90 mg/kg）. Escape latency and times of running across the platform were respectively determined by Morris water maze. The activity of serum superoxide dismutase （SOD） , glutathione peroxide （GSH-Px） and the content of malondialdehyde （MDA） were detected with biochemical enzyme and thiobarbituric acid. Morphology of neurons in the hippocampal CA1 region was observed with H＆E staining. Results When compared with that of the control group, the escape latency for the model group obviously prolonged [ （76.13±25.28 ） s ] （ P 〈 0.01 ） , times of running across the platform reduced [ （ 2.34 ±0.87 ） timesJ（P〈0.01）, the activity of SOD [（269.70±33.45）U/ml] and GSH-Px [（4.38 ± 1.21）g/L] significantly decreased, but the content of MDA increased [ （6.57± 2.04）μmol/L] （P 〈0. 01 ）. A great amount of degenerative neurons and sparse irregular cell arrangements could be detected in hippocampal CA1 region in the dementia mice. Following HBO treatment, the escape latency shortened, the times of running across the platform increased, the activity of SOD and GSH-Px significantly increased, while the content of MDA decreased, with statistical significance （P 〈 0.01 ）. The No. of normal cells in the hippocampal CA1 region increased significantly, when compared with that of HBO treatment, and the neurons in the CA1 region were tightly arranged. Conclusions HBO could obviously increase the capacity of the mice with AD to alleviate lesion induced by oxygen free radicals and has certain effect on the treatment of AD through anti-senile mechanism.

关 键 词：高压氧 老年痴呆 超氧化物歧化酶 丙二醛 谷胱甘肽 氧化应激 

分 类 号：R285.5[医药卫生—中药学]