高原低氧环境下骨质疏松大鼠模型的骨代谢生化指标观察  

Observation of biochemical indexes of bone metabolism in ovariectomized rats with osteoporosis at high altitude

作　　者：高文魁[1] 白峰[1] 王伟[1] 闫自强[1] 王德元[1] 李智刚[1] 马力夫[1] 

机构地区：[1]解放军第四医院骨科,青海西宁810007

出　　处：《中国骨质疏松杂志》2011年第11期941-944,共4页Chinese Journal of Osteoporosis

摘　　要：目的观察高原低氧环境下骨质疏松大鼠模型的骨形成相关生化指标变化,探讨其在高原地区骨质疏松症诊断和监测中的应用。方法选用纯种SD雌性大白鼠72只,随机分为A组(高原去势组)、B组(平原去势组)、C组(高原对照组)和D组(平原对照组)。术后分别在海拔高于3000米的高原和低于500米的平原饲养。并于术后4周、12周和24周测量大鼠的股骨骨密度和血清相关生化指标。结果 12周时,高原和平原饲养的去势大鼠骨密度下降较为显著(P<0.05),且高原去势组骨密度明显低于平原去势组(P<0.05),对照组均无显著变化(P>0.05)。24周,除平原对照组骨密度无显著变化,其它三组骨密度均有显著降低,且高原去势组骨密度明显低于其它组,差异具有统计学意义(P<0.01)。生化指标检测发现,高原饲养组与平原饲养组比较,血清钙浓度无显著性差异(P>0.05)。血清磷离子的含量由高到低为平原对照组、高原对照组、平原去势组和高原去势组,差异具有统计学意义(P<0.05)。碱性磷酸酶在各时间段及各组之间均无明显差异(P>0.05)。不论是去卵巢还是未去卵巢,与平原饲养的大鼠相比,高原饲养的大鼠的血清骨钙素含量始终维持在低水平(P<0.01)。去势组大鼠的血清激素雌二醇的含量显著低于对照组,而饲养于高原后,这种差异更加显著(P<0.01)。结论高原缺氧环境可引起骨代谢异常,并加速骨质疏松发展的进程,联合应用骨密度及血清骨形成生化指标检测有助于高原骨质疏松症的诊断。Objective To observe the biochemical indexes of bone metabolism in ovarieetomized rats with osteoporosis at high altitude and explore the application of biochemical indexes of bone metabolism in the diagnosis and monitoring of osteoporosis at high altitude. Methods 72 female SD rats were chosen and divided into group A （ovariectomized group at high altitude） , group B （ovariectomized group at low altitude） , group C （normal group at high altitude） and group D （normal group at low altitude）. After operation, the rats were bred at high altitude and low altitude respectively. The bone mineral density of the femoral bone and serum biochemical indexes were determined at 4,12,24 weeks. Results At 12 weeks after ovariectomy, the bone mineral density of ovariectomized rats was significantly decreased compared with the normal group（P 〈 0.05）. The bone mineral density of ovariectomized group at high altitude was lower than that of ones at low altitude（P 〈 0.05）. Twenty-four weeks after operation, the bone mineral density of ovarieetomized group at high altitude was significantly lower than that in other groups （ P 〈 0. 01 ）. Detection of the bioehemieal indexes of bone metabolism： There were no obvious differences in serum calcium and alkaline phosphatase among the groups at high ~altitude and the groups at low altitude at each time point（P 〉 o. 05 ）. The order of the serum phosphorus from high to low was group D,C, B and A. The serum osteocalcin and estradiolum in groups at high altitude was significantly lower as compared with those at low altitude group（ P 〈0. 01 ）. Conclusion It suggested that the effect of oxygen deficit at high altitude might cause abnormal bone metabolism and accelerate the evolution of osteoporosis. The application of the biochemical indexes of bone metabolism together with the bone mineral density is helpful for the diagnosis of osteoporosis at high altitude.

关 键 词：高原 低氧 骨质疏松 骨代谢 

分 类 号：R683[医药卫生—骨科学]