胃肠道间质肿瘤DOG1和Ki67、p53的表达及意义  

The Expression and Clinical Significance of DOG1 and Ki67, p53 in Gastrointestinal Stromal Tumors

作　　者：郑铭[1,2] 江淑萍[1,3] 余惠珍[1,2] 

机构地区：[1]福建省立医院 [2]福建医科大学省立临床医学院干部特诊二科,福州350001 [3]福建医科大学省立临床医学院病理科,福州350001

出　　处：《福建医科大学学报》2011年第5期351-354,共4页Journal of Fujian Medical University

摘　　要：目的探讨胃肠道间质瘤(GIST)患者中的DOG1和细胞增殖核抗原Ki67以及p53突变型蛋白的相互关系及病理学意义。方法应用免疫组织化学方法检测86例GIST患者与20例非GIST患者组织中的DOG1及Ki67、p53的表达,比较分析各个指标在GIST各危险性分级和各部位的表达情况。结果在86例GIST组织中,DOG1与CD117的表达阳性率间比较差别无统计学意义(97.7%vs 90.7%,P>0.05);而CD117表达阴性的GIST组织中,DOG1阳性表达率为87.5%。GIST经危险性分层后,DOG1阳性表达率在高、中、低、极低度危险组中比较差别无统计学意义(P>0.05)。Ki67和p53在GIST组织中的阳性表达率分别为58.1%和47.7%;Ki67和p53在高度和中度危险性组的表达阳性率显著高于低度和极低度危险性组。GIST组织的DOG1、CD117、Ki67和p53阳性表达率显著增高于非GIST组织(P<0.01)。结论 DOG1联合CD117可作为诊断GIST或鉴别非GIST的一个敏感而特异的重要标记物;Ki67以及突变型p53蛋白可作为判断GIST良恶性的生物学指标以及预后判断的重要标志。Objective To investigate the protein expression level of DOG1 and Ki67,p53 in differrent biological aggressive risk of gastrointestinal stromal tumors（GIST） and potentially clinical significance.Method The expressions of DOG1 and Ki67,p53 were analyzed by immunohistochemical staining method in 86 cases of GIST and 20 cases of no-GIST.At the same time,the relationship between GIST and no-GIST was compared.Results Positive percentages for DOG1,Ki67,and p53 were 97.7%,58.8% and 47.7% respectively in 86 cases of GIST.There was no significant difference between the expression positive percentage of DOG1 and CD117（97.7% vs 90.7%,P0.05）.The negative experssion of CD117 in 8 cases showed that the positive expression percentage of DOG1 was 87.5%.No significant correlation was observed between DOG1 expression and the risks tratification of the tumor and tissue site of the patient（P0.05）.Positive percentages for Ki67 and p53 were higher in group of high and intermediate risk than that in group of low and very low risk（P0.05,P0.01）.There were significant difference between positive percentages for DOG1,Ki67and p53 in GIST and no-GIST（P0.001）.Conclusions DOG1 protein was very high expression in GIST.Positive DOG1 staining combined with CD117 positivity enhances further a diagnosis for GIST and GIST of CD117 negative-staining.The expression of p53 and Ki67 may be an important marker in the diagnosis of risk tratification and prognosis in gastrointestinal stromal tumors.

关 键 词：胃肠道间质肿瘤 KI67抗原 基因 p53 原癌基因蛋白质C-KIT 肿瘤标记 生物学 免疫组织化学 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R392.112[医药卫生—基础医学]