人源重组ICOSIg对鼠不成熟树突状细胞的生物学作用  

Biological effect of human-derived recombinant ICOSIg on mouse immature dendritic cells

作　　者：徐劲松 吴豫 沈茜[2] 唐古生[2] 

机构地区：[1]解放军94医院呼吸科,南昌330002 [2]第二军医大学长海医院实验诊断科,上海200433

出　　处：《第二军医大学学报》2011年第11期1165-1170,共6页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金青年基金(81102249);中国人民解放军南京军区2010年医学课题(10MA057);江西省卫生厅2011年度科技计划项目(20112058)~~

摘　　要：目的分析人源可诱导共刺激分子(ICOS)可溶性融合蛋白(ICOSIg)与鼠源不成熟树突状细胞(DCs)是否发生特异性结合及其一般生物学特性。方法通过流式细胞术结合特异性抗体检测了解ICOSIg与不成熟DCs的结合作用;以AnnexinⅤ/PI双染色、活细胞染料CSFE和CCK-8研究ICOSIg对DCs的细胞毒性作用;以[3 H]掺入法研究ICOSIg对于混合淋巴细胞反应的阻断作用。结果 ICOSIg可结合小鼠DCs表面的ICOSL,ICOSIg不引起DCs早期和晚期凋亡,不影响DCs细胞的增殖,可以抑制不同基因小鼠脾细胞的混合淋巴细胞反应。结论本实验室构建的体外表达的ICOSIg具有较强的生物学活性,对鼠DCs无明显的生物学毒性作用,首次从实验角度证实人源ICOSIg可以特异性结合小鼠不成熟DCs表面配体ICOSL,可以用于进一步探讨ICOSL/ICOS共刺激通路的免疫作用。Objective To analyze whether human-derived ICOSIg can bind specifically to ICOSL on mouse immature dendritic cells（DCs） and to explore its biological functions.Methods The binding of ICOSIg to immature DCs was observed by FCM.The cytotoxic effect of ICOSIg on DCs was examined by Annexin Ⅴ/PI,CFSE staining,and CCK-8 kit. thymine incorporation was used to analyze the blocking effect ICOSIg on mixed lymphocyte reaction（MLR）.Results Human-derived soluble fusion protein ICOSIg could bind to ICOSL on mouse bone marrow-derived immature DCs and inhibited the MLR,but it neither induced early or late apoptosis of DCs nor affected their proliferation.Conclusion Human-derived ICOSIg constructed in this study has a potent biological function;it has no toxic effect against mouse immature DCs.It is demonstrated that human-derived ICOSIg can sepecifically bind to ICOSL on mouse immature DCs.

关 键 词：可诱导共刺激分子 树突细胞 重组融合蛋白质类 抑制 

分 类 号：R392.111[医药卫生—免疫学]