梗阻性黄疸对肾脏水通道蛋白-3表达影响的实验研究  

Experimental Research of Renal Aquaporin-3 Expression in Obstructive Jaundice

作　　者：张德巍[1] 关维雨[1] 刘金钢[2] 王勇[2] 

机构地区：[1]中国医科大学附属第四医院急诊外科,辽宁沈阳110032 [2]中国医科大学附属盛京医院肝胆外科,辽宁沈阳110005

出　　处：《中国普外基础与临床杂志》2011年第11期1175-1179,共5页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的通过检测梗阻性黄疸(OJ)时大鼠肾脏水通道蛋白-3(AQP-3)基因及蛋白表达的变化,探讨其在早期肾功能损害诊断中的价值。方法将Wistar大鼠40只,随机分为实验组(n=20,建立OJ动物模型)和对照组(n=20,假手术组)。于术后7d和14d,采用全自动生化分析仪检测各组大鼠血总胆红素(TBIL)、直接胆红素(DBIL)、肌酐(Cr)和尿素氮(BUN)水平,并用Western blotting法和RT-PCR法分别检测各组大鼠肾脏AQP-3蛋白和mRNA的表达水平。结果 实验组大鼠术后14d血清TBIL和DBIL水平明显高于术后7d(P=0.000),且均高于相应时相假手术组(P=0.000),而假手术组内两时相间比较差异无统计学意义(P=0.154),证实OJ模型建立成功。血Cr水平在2组内不同时相间和相同时相组间比较差异均无统计学意义(P>0.05)。实验组大鼠术后14d的血BUN水平明显高于术后7d以及假手术组术后14d的水平(P=0.001),而假手术组术后两时相间的差异无统计学意义(P=0.288)。实验组大鼠术后7d及14d的AQP-3蛋白表达水平均明显低于相应时相的假手术组(P=0.033,P=0.000),且术后14d的水平又明显低于同组术后7d水平(P=0.000);假手术组大鼠术后两时相的AQP-3蛋白表达水平间的差异无统计学意义(P=0.059)。实验组大鼠术后7和14d的AQP-3mRNA表达水平明显高于相应时相的假手术组(P=0.000),而两组内不同时相间比较,其差异均无统计学意义(P=0.139,P=0.059)。结论 OJ合并肾功能损害时大鼠肾脏AQP-3mRNA及蛋白表达的变化早于血肌酐及尿素氮的变化,其变化的应用有望提高OJ时肾功能损害的早期诊断率。Objective To explore the value of Aquaporin-3（AQP-3） on the detection of early renal function damage by investigating the expressions of renal AQP-3 mRNA and protein of rats with obstructive jaundice（OJ）.Methods Forty mature male Wistar rats were divided into two groups randomly： experimental group（n=20） in which the model of OJ rats was established,and control group（n=20,sham operation group）.The levels of serum total bilirubin（TBIL）,direct bilirubin（DBIL）,creatinine（Cr）,and blood urea nitrogen（BUN） were detected by full-automatic biochemical analyzer on 7 d and 14 d after operation.The expressions of renal AQP-3 mRNA and protein of rats were detected by RT-PCR and Western blotting,respectively.Results The levels of serum TBIL and DBIL were significantly higher on 14 d than those on 7 d after operation in experimental group（P=0.000）,which were significantly higher than those at corresponding time point in control group（P=0.000）,while the difference within control group was not significant（P=0.154）.Thus,the OJ models of rats were established successfully.The difference of serum Cr levels of rats between inter-and intra-group were not significant（P0.05）.Serum BUN level on 14 d after operation in experimental group was significantly higher than those on 7 d after operation in experimental group and on 14 d after operation in control group（P=0.001）,although serum Cr levels were not different between 7 d and 14 d after operation in control group（P=0.288）.The expressions of AQP-3 protein of rats on 7 d and 14 d after operation in experimental group were significantly lower than those at corresponding time point in control group（P=0.033,P=0.000）,meanwhile on 14 d after operation in experimental group was significantly lower than those on 7 d after operation in experimental group（P=0.000）.The expressions of AQP-3 mRNA of rats on 7 d and 14 d after operation in experimental group were significantly higher than those at corresponding time point in control g

关 键 词：梗阻性黄疸 急性肾功能衰竭 水通道蛋白-3 大鼠 

分 类 号：R657.4[医药卫生—外科学]