碳纳米管对单核巨噬细胞作用的基因表达谱研究  

Gene Expression of Multi-Walled Carbon Nanotubes on Macrophages

作　　者：罗蔓[1] 邓小勇[2] 董玲[1] 沈锡中[1] 

机构地区：[1]复旦大学附属中山医院,上海200032 [2]上海大学纳米化学与生物学研究所,上海200444

出　　处：《中国临床医学》2011年第5期599-602,共4页Chinese Journal of Clinical Medicine

摘　　要：目的:采用Affmetrix公司的基因组芯片,研究原始的多壁碳纳米管(pristine multi-walled carbon nanotubes,p-MWC-NTs)与牛磺酸修饰的多壁碳纳米管(taurine functionalized multi-walled carbon nanotubes,tau-MWCNTs)对小鼠的单核巨噬细胞RAW264.7细胞的作用机制。方法:通过对差异表达基因的生物学功能予以分类,从分子水平上探讨p-MWCNTs与tau-MWCNTs对RAW264.7细胞的作用机制,并以实时荧光定量聚合酶链反应来验证研究结果。结果:与对照组相比,p-MWCNTs处理RAW264.7细胞24 h后,RAW264.7细胞表达显著改变的基因共5 738个;而经tau-MWCNTs处理RAW264.7细胞24 h后,RAW264.7细胞表达显著改变的基因共2 567个,这些基因的功能涉及细胞周期、细胞凋亡、应激反应、炎性反应与细胞骨架等。结论:P-MWCNTs抑制RAW264.7细胞的生长是通过调控一些与细胞周期、细胞凋亡、应激反应及炎性反应相关的基因而起作用。而tau-MWCNTs处理RAW264.7细胞后发生变化的基因数量显著减少。Objective：Using Affymetrix Genechip, we had studied the possible mechanism of p-MWCNTs and tau-MWCNTs on RAW264.7 macrophages. Methods： Changes of gene expression in RAW264.7 macrophages, after treatment with p-MWCNTs and tau-MWCNTs, were examined by the cDNA microarray in order to study the molecular mechanism. Real time RT-PCR was conducted to verify the differential expression of some genes. Results： After the RAW 264.7 macrophages were ex- posed to the p-MWCNTs for 24h, compared with the control, there were 5738 genes showed differential expression; On the other hand, after the RAW 264.7 macrophages were exposed to the tau-MWCNTs for 24h, there were 2 567 genes showed differential expression. According to the Gene Ontology, these genes were involved in cell cycle, apoptosis, stress and inflammation, cytoskeleton and so on. Conclusions： The p-MWCNTs inhibit the proliferation of the RAW 264.7 macrophages by regula- ting the genes expression, including cell cycle, apoptosis, stress, inflammation and so on. There are reduced differential expression genes on the RAW 264.7 macrophages after treatment with the tau-MWCNTs.

关 键 词：多壁碳纳米管 RAW264.7细胞 凋亡 基因芯片 

分 类 号：R318[医药卫生—生物医学工程]