高效液相色谱法测定水样中莠去津方法的改进  

Study on improved determination method of atrazine in water by HPLC

作　　者：王若燕[1] 周晓萍[1] 杜赛 

机构地区：[1]浙江省绍兴市疾病预防控制中心,浙江绍兴312000

出　　处：《中国卫生检验杂志》2011年第11期2645-2646,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:研究高效液相色谱法测定水样中莠去津的最佳方法。方法:用二氯甲烷萃取水样中的莠去津,浓缩,挥干,甲醇定容后用高效液相色谱仪测定。色谱条件:waters Atlantis C18柱(4.6×250 mm,4.6μm);流动相:甲醇/水(60/40);二极管阵列检测器,检测波长220 nm。结果:在0.05μg/ml～5.0μg/ml范围内有良好线性关系,r=0.9999。最低检测质量0.5 ng,样品最低检测浓度为0.0005 mg/L。相对标准差3.6%～4.2%,回收率88%～91%。结论:用该方法测定水样中的莠去津,能获得满意的结果。Objective：To establish a better HPLC method for the determination of atrazine in water.Methods： The sample was extracted by dichloromethane,condensed,then melted in methanol,and at last analyzed by HPLC.The test sample was separated by gradient elution with methanol（60%） and water（40%） on Atlantis C18 and determined by diode-array detector at wavelength of 220 nm.Results：This HPLC method exhibited a linear relation within 0.05 μg/ml^5.0 μg/ml,r=0.9999.The minimal detectable quality of this method was 0.5 ng,and the minimal detectable concentration was 0.0005 mg/L.The recoveries of the method ranged from 88% to 91%,with relative standard deviations of 3.6%~4.2%.Conclusion： This method can be used to determine atrazine in water with good results.

关 键 词：莠去津 高效液相色谱法 水样 

分 类 号：O657.72[理学—分析化学]