女性维生素D受体基因FokI遗传多态性对维生素D受体蛋白的影响  

Effect of vitamin D receptor(VDR) FokI polymorphism on VDR protein levels in females

作　　者：黄友泽 白永恒[2] 陆红[2] 曾云祥 陈以勒 潘贻飞[2] 

机构地区：[1]温州市第八人民医院,浙江温州325000 [2]温州医学院附属第一医院,浙江温州325000

出　　处：《中国卫生检验杂志》2011年第11期2657-2658,2661,共3页Chinese Journal of Health Laboratory Technology

基　　金：温州市鹿城区科技计划项目(S10106)

摘　　要：目的:探讨女性人群维生素D受体(VDR)基因5'端转录起始位点FokI多态性对VDR蛋白水平的影响。方法:以229例女性人群作为研究对象,采用PCR-RFLP检测其VDR基因FokI位点多态性,ELISA检测血浆VDR蛋白水平。结果:FokI位点基因型符合H-W平衡检验;基因型以杂合子Ff为主,占50.26%;FF基因型女性的血浆VDR水平为82.67±34.14 pmol/ml,显著低于Ff(103.78±17.48 pmol/ml)或ff基因型(114.73±53.00 pmol/ml);VDR水平与年龄明显相关。结论:女性人群VDR基因FokI多态性与血浆VDR蛋白水平相关,FF基因型具有较低的VDR水平。Objective：To explore the effect of FokI polymorphisms of the VDR gene on VDR protein levels in females.Methods： A total of 229 females were enrolled in our study,and the genotypes were detected by Polymerase chain reaction-restrained fragment length polymorphism technique（PCR-RFLP）.The levels of VDR protein were measured by enzyme-linked immunosorbent assay（ELISA）.Results： The frequency of FokI genotype followed the Hardy-Weinberg equilibrium test.The distribution of FokI Ff genotype is most,and the frequency was 50.26%.Plasma VDR levels in females with FF genotype was 82.67±34.14 pmol/ml,significantly lower than that in females with Ff（103.78±17.48 pmol/ml） or ff genotype（114.73±53.00 pmol/ml）.Additionally,the VDR levels also showed an association with age.Conclusion： The FokI polymorphism of VDR gene was associated with plasma VDR levels in females,and those with FF genotype showed lower plasma VDR levels.

关 键 词：维生素D受体 基因型 PCR-RFLP ELISA 

分 类 号：Q343.1[生物学—遗传学]