应用脉冲场凝胶电泳对伤寒沙门菌分型研究  

The application of PFGE in the typing of salmonella

作　　者：陆剑波[1] 陈丽[2] 程均福[2] 

机构地区：[1]湖北省咸宁市疾病预防控制中心,湖北咸宁437100 [2]湖北省疾病预防控制中心,武汉430070

出　　处：《中国卫生检验杂志》2011年第11期2696-2697,2700,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:运用脉冲场凝胶电泳(PFGE)技术对湖北2008年分离的沙门菌菌株进行分析,探讨湖北沙门菌的分布、流行及变异情况。方法:对分离的沙门菌利用全自动生化分析系统、血清分型、及脉冲场凝胶电泳(PFGE)分子生物学技术进行研究。结果:18份测试株用XbaI酶切后产生3个带型。结论:通过脉冲场凝胶电泳(PFGE)全基因DNA指纹图谱分析,可追溯其同源性。通过18株伤寒菌株的PFGE分型图谱分析,其中相似系数100%15份,占83.33.%,可确认为相同病原菌感染。由此说明PFGE是一种灵敏、可靠的研究沙门菌分子流行病学基因分型方法。Objective：To explore the distribution,popularity and variation of salmonella in Hubei through analyzing the salmonella strains isolated in 2008 by pulsed field gel electrophoresis（PFGE）.Methods： Biochemical full autoimmunization analyzing system,typing of blood serum,typing of drug sensitivity and PFGE molecular biology technology were used to make a research on separated salmonella.Results： 18 tested strains produced 3 banding patterns after being divided by enzyme XbaI.Conclusion： The homology of salmonella of Hubei in 2008 can be traced by analyzing the whole genome DNA fingerprint by PFGE.Through analysis of PFGE typing map among the18 typhoid strains,15 strains（83.33%） are found complete homology,which confirms that they are infected by the same pathogenic bacteria.So PFGE is a sensitive and reliable method to study salmonella genotyping in molecular epidemiology.

关 键 词：沙门菌 PFGE 电泳 

分 类 号：R446.5[医药卫生—诊断学]