2010年福建南平及永安监测点致泻大肠杆菌的监测报告  

Surveillance of diarrheagenic Escherichia coli infection in Nanping and Yongan monitoring sites of Fujian in 2010

作　　者：林杰[1] 陈爱平[1] 陈建辉[1] 李玉燕[2] 杨劲松[1] 郑金凤[1] 

机构地区：[1]福建省疾病预防控制中心,福州350001 [2]福建省南平市延平区疾病预防控制中心,福建南平353000

出　　处：《中国卫生检验杂志》2011年第11期2722-2725,共4页Chinese Journal of Health Laboratory Technology

基　　金：福建省医学创新课题(2011-CXB-19)

摘　　要：目的:为了解福建省腹泻病人中致泻大肠杆菌的感染情况。方法:应用多重PCR或单重PCR方法检测致泻大肠杆菌的EPEC/EHEC eaeA基因、EHEC stx基因、EAEC aggR基因、EIEC ipaH、EIEC virA、ETEC ST、ETEC LT、EPEC bfp基因。API 20E生化鉴定条进行生化试验。血清学鉴定。结果:2010年度共分离得19株致泻大肠杆菌,检出率为10.9%。1株EAEC;1株tEPEC;4株aEPEC;13株ETEC。19株分离的致泻大肠杆菌经API 20E鉴定均为大肠埃希菌。1株tEPEC与EPEC三种多价诊断血清型均不凝集,而1株aEPEC血清型为O111:K58(B4)。13株ETEC菌株血清型:多数为O6:K15,1株O25:K19(L),3株未能分型。结论:监测结果对于我们了解福建省致泻大肠杆菌感染情况,预警潜在发生疫情的可能性有其重要性。这些菌种的获得可以为下一步研究其毒力特征、分子分型及耐药性积累原始材料。Objective：To understand the condition of Diarrheagenic Escherichia coli（DEC） infection in Fujian province.Methods： Genes such as EPEC/EHEC eaeA,EHEC stx,EAEC aggR,EIEC ipaH,EIEC virA,ETEC ST,ETEC LT,EPEC bfp were detected by multiplex or single PCR.Biochemical identification was determined by API 20E strip and serotyping was performed.Results： 19 strains of DEC（1 EAEC,1 tEPEC,4 aEPEC and 13 ETEC） were isolated in the surveillance in 2010,and the detection rate was 10.9%.All isolated strains were identified as E.coli by API 20E strip.1 tEPEC didn′t agglutinate with EPEC multivalence diagnostic serum,whereas 1 aEPEC agglutinated with EPEC serum O111：K58（B4）.Serotypes of 13 ETEC were mainly O6：K15,while the others were 1 strain for O25：K19（L）,3 strains untyped.Conclusion： The surveillance is important to understand DEC infection in Fujian province and early warn the potential of DEC epidemic.The isolated strains provide material for further study of their virulence characteristics,molecular typing and antibiotic resistance.

关 键 词：EAEC EHEC EIEC EPEC ETEC 

分 类 号：R378.21[医药卫生—病原生物学]