超临界CO_2流体萃取侧柏叶萜烯化合物方法的讨论  

Supercritical CO_2 fluid extraction of terpenoids in platycladus orientalis leaves

作　　者：王骏[1] 吴京科[1] 

机构地区：[1]北京林业大学,北京100083

出　　处：《分析仪器》2011年第6期65-67,共3页Analytical Instrumentation

摘　　要：在改变温度和压力条件下尤其是添加夹带剂后,超临界CO2流体可进一步增加侧柏叶萜烯类化合物的萃取量和化合物种类。本文通过控制单因子变化,选取无水乙醇和乙酸乙酯为夹带剂,对侧柏叶中萜烯类化合物α-蒎烯、β-石竹烯进行萃取和GC、GC/MS检测。研究结果表明,应用体积10mL的萃取釜,在鲜侧柏叶1.5g,萃取温度为38℃、压力为30MPa时,2mL无水乙醇对侧柏叶α-蒎烯、β-石竹烯的提取率较好。Adding entrainer in supercritical CO2 fluid will increase the extraction quantity and extracted types of terpenoids in platycladus orientalis leaves. In this paper, terpenoids α-pinene and β-caryophyllene in platy-cladus orientalis leaves were extracted through controlling the single factor and by using ethanol and ethyl acetate as entrainers, and were detected by GC and GC/MS. The results showed that using an extraction vessel with a volume of 10mL, the optimal extraction rate for α-pinene and β-caryophyllene was achieved when 2mL ethanol was used as entrainer and the fresh platycladus orientalis leaves was 1.5g, with the ex-traction temperature of 38℃ and the extraction pressure of 30 MPa.

关 键 词：超临界CO2流体萃取 夹带剂 萜烯类化合物 侧柏叶 

分 类 号：TQ28[化学工程—有机化工]