皇冠果壳中皇冠苷A的提取工艺优化及含量测定  

Optimization of the Extraction Technology and Determination of Mahkoside A in Nut Shell of Mahkota dewa

作　　者：张雁冰[1] 张全海[1] 张赛扬[1] 马刚[1] 毕跃峰[1] 符玲[1] 

机构地区：[1]郑州大学药学院新药研究开发中心,河南郑州450001

出　　处：《郑州大学学报（理学版）》2011年第4期77-80,共4页Journal of Zhengzhou University:Natural Science Edition

摘　　要：用HPLC方法测定了皇冠果壳中皇冠苷A的含量,确定了HPLC法测定皇冠苷A含量的条件.测定结果为:皇冠果壳中皇冠苷A的含量不低于16.47 g/kg.用正交试验优化了皇冠果壳中皇冠苷A的提取工艺,结果表明其理论最佳提取工艺条件为:用体积分数55%的乙醇提取2次,每次提取1 h,每次所用乙醇的体积为皇冠果壳质量的20倍.The content of Mahkoside A in nut shell of Mahkota dewa was determined by HPLC method.The results indicated that the content of Mahkoside A was not less than 16.47 g/kg.The extraction process for Mahkoside A in nut shell of Mahkota dewa was selected through orthogonal test,and the optimum extraction process was as follows： adding 20 times amount of 55% alcohol into medical materials,and refluxing and extracting for 2 times,each time for 1 h.

关 键 词：HPLC 皇冠苷A 正交试验 工艺优化 含量测定 

分 类 号：R284.2[医药卫生—中药学] R927.2[医药卫生—中医学]