鸭产蛋下降-死亡综合征的临床诊断与病原的初步鉴定  

Clinical diagnosis and pathogenic identification of egg drop-death syndrome in duck

作　　者：赵圆圆[1] 李传峰[2] 朱良强[3] 詹松鹤[3] 王汉清[1] 魏建忠[1] 秦爱建[4] 刘光清[2] 王桂军[1] 

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230036 [2]中国农业科学院上海兽医研究所,上海200241 [3]安徽省动物疫病预防与控制中心,安徽合肥230031 [4]扬州大学兽医学院,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2011年第3期11-14,共4页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家高技术研究发展计划项目(863-2011AA10A200);公益性行业(农业)科研专项(201003012)

摘　　要：2010年3～11月间,安徽省部分规模养鸭场流行以产蛋下降和死亡为特征的急性、热性鸭传染病,主要感染成年种鸭或蛋鸭,病理变化则以心肌出血、卵巢肿大、出血和坏死为特征。通过鸡胚接种、传代培养和RT-PCR鉴定,从发病鸭体内分离1株黄病毒。对其NS1基因扩增并测序,发现其与坦布苏病毒亲缘关系非常近,核苷酸同源性达94.2%。动物回归试验结果表明:该病毒能引起蛋鸭产蛋下降和卵巢病变。证明鸭黄病毒在我国对鸭的高度致病性,并开始在规模鸭场蔓延和流行,应当引起有关部门和兽医工作者的高度重视。During March to November of 2010,a kind of duck disease,which was characterized by egg drop and death syndrome,occurred in large-scale duck farms in Anhui province.A strain of flavivirus was isolated by chicken embryo inoculation and RT-PCR from the infected ducks,which experienced the gross lesions with myocardial hemorrhage,ovarian enlargement and necrosis.Animal regression experiment was conducted for identification of pathogenicity of virus isolate.The results suggested that this disease was caused by a new duck flavivirus that was closely related to Tembusu virus,which shared 94.2% sequence homology at NS1 nucleotide level.This study further confirmed that duck flavivirus was highly pathogenic to duck in China and began to spread in large scale duck farms,and it is necessary to attach great importance to duck flavivirus infection.

关 键 词：产蛋下降-死亡综合征 坦布苏病毒 黄病毒 病原鉴定 

分 类 号：S858.32[农业科学—临床兽医学] S855.3[农业科学—兽医学]