Measuring methods for three kinds of polyhydroxyalkanoates from activated sludge by gas chromatography  

作　　者：陈玮 陈志强 吕炳南 温沁雪 张运海 

机构地区：[1]State Key Lab of Urban Water Resource and Environment,School of Municipal and Environmental Engineering,Harbin Institute of Technology [2]National Engineering Research Center of Urban Water Resources

出　　处：《Journal of Harbin Institute of Technology(New Series)》2011年第5期113-118,共6页哈尔滨工业大学学报（英文版）

基　　金：Sponsored by the National Water Plan(Grant No.2008ZX07317-008);the Stake Key Lab of Urban Water Resource and Environment(Grant No.2010DX02)

摘　　要：Gas chromatography determination of polyhydroxyalkanoates has been common;however,the pretreatment steps are often complex,and gas chromatography operation conditions are not given in detail.In this study,gas chromatography is used for analyzing PHB,PHV and PH2MV,three majors of PHAs in activated sludge.The sample was centrifuged at a speed of 4000 r/min for the separation of floc and supernatant,freezen,and dried for 12 h in vacuum freezing drier;and then transferred to the fridge for freezing to ice and drying for 12 h in vacuum freezing drier;then chloroform and a simple composition digestion solution including methanol,sulfuric acid and benzoic acid was added;digested at 105 ℃ for 6 h;cooled to room temperature,the lower solution of the result can be used for analyzing.Samples were analyzed by gas chromatography with FID detector and auto sampler;the standard curve of standard material shows an excellent linear relationship with correlation coefficients larger than 0.99;the relative standard deviation (RSD) of sludge samples is less than 1%.The recovery rates of each sample are between 95%-105%.The GC analysis time of each PHA sample is shorter than 10 minutes.Gas chromatography determination of polyhydroxyalkanoates has been common; however, the pretreatment steps are often complex, and gas chromatography operation conditions are not given in detail. In this study, gas chromatography is used for analyzing PHB, PHV and PH2MV, three majors of PHAs in activated sludge. The sample was centrifuged at a speed of 4000 r/min for the separation of floe and supernatant, freez- en, and dried for 12 h in vacuum freezing drier; and then transferred to the fridge for freezing to ice and drying for 12 h in vacuum freezing drier; then chloroform and a simple composition digestion solution including metha- nol, sulfuric acid and benzoic acid was added; digested at 105 ~C for 6 h; cooled to room temperature, the low- er solution of the result can be used for analyzing. Samples were analyzed by gas chromatography with FID detector and auto sampler; the standard curve of standard material shows an excellent linear relationship with cor- relation coefficients larger than 0. 99 ; the relative standard deviation （RSD） of sludge samples is less than 1%. The recovery rates of each sample are between 95% - 105%. The GC analysis time of each PHA sample is shorter than 10 minutes.

关 键 词：POLYHYDROXYALKANOATES activated sludge gas chromatography measuring method 

分 类 号：X703.1[环境科学与工程—环境工程]