鳜早期发育阶段骨骼肌纤维的增生与肥大生长  

Hyperplastic and Hypertrophic Growth of Skeletal Muscle Fibers of Mandarin Fish(Siniperca chuatsi) at Early Developmental Stage

作　　者：朱琼[1] 赵金良[1] 苌建菊[1] Jeerawat THAMMARATSUNTORN 钱叶洲 吴超 钱德 

机构地区：[1]上海海洋大学农业部淡水水产种质资源重点实验室,上海201306 [2]安徽省池州市特种水产研究所,池州247100

出　　处：《动物学杂志》2011年第6期96-102,共7页Chinese Journal of Zoology

基　　金：上海市科委重点基础项目(No.09JC1406900);上海市重点学科建设项目(No.Y1101)

摘　　要：骨骼肌是鱼体的主要组成部分,也是衡量鱼体生长发育的重要指标。为了解鳜(Siniperca chuatsi)早期发育阶段骨骼肌纤维的生长发育特征,通过制作孵化后1～41日龄个体骨骼肌背右侧第一肌节石蜡切片,利用图像分析软件统计该肌节中肌纤维的数目和面积,分析了鳜骨骼肌纤维的增生和肥大生长特征。结果表明,鳜早期发育阶段骨骼肌生长同时包括数目增加(增生)和面积增大(肥大),背侧第一肌节中肌纤维总数由40个增加到520个,肌纤维总面积由805.30μm2增加到186 422.77μm2。其中,孵化后1～9日龄,肌纤维相对增生数目下降,相对增加面积缓慢上升,相对增加面积中增生生长的贡献率由60.53%降至8.80%,肥大生长则与之相反;9～15日龄,肌纤维相对增生数目呈现上升,相对增加面积相对平缓,相对增加面积中增生生长的贡献率略呈上升(由8.80%上升至20.26%),肥大生长贡献率略有下降;15～41日龄,相对增生数目和相对增加面积均呈上升趋势,相对增加面积中增生生长的贡献率下降并趋于平稳,肥大生长贡献率由79.79%上升到87.41%,成为优势生长。Skeletal muscle is the main composition to body weight in fish,which provides an important indicator for somatic growth.To fully understand skeletal muscle development of mandarin fish（Siniperca chuatsi） at the early stage,paraffin sections of the first dorsal myomere of skeletal muscle at 1-41 days after hatching were carried out;fiber numbers and total fibre area were measured to describe hyperplastic and hypertrophic growth of skeletal muscle.The results showed that skeletal muscle growth of mandarin fish included increasing of both fibre numbers（hyperplasia） and fibre area（hypertrophy） at the early development stage,the total fiber numbers in the right first dorsal myomere increased from 40 to 520,and the total fiber area increased from 805.30 μm2 to 186 422.77 μm2.During the 1-9 days after hatching,the relative increased fiber numbers decreased and the relative increased fibre area raised slowly,the contribution rate of hyperplastic growth dropped dramatically from 60.53% to 8.80%,as opposite to hypertrophic growth.At the period of 9-15 days after hatching,the relative increased fiber numbers raised and relative increased area was on a steady phase,the contribution rate of hyperplastic growth increased from 8.80% to 20.26%,while hypertrophic growth contribution ratio decreased slightly.At the 15-41 days after hatching,both the relative increased fiber numbers and area were on the rise,the contribution rate of hyperplastic growth decreased to a steady phase and hypertrophic growth（from 79.76% to 87.41%） gradually became the dominant contributor of skeletal muscle growth.

关 键 词：鳜 骨骼肌纤维 增生生长 肥大生长 贡献率 

分 类 号：S917.4[农业科学—水产科学]