麻黄素对仔鼠肾组织结构和SOD、CAT活力及MDA含量的影响  

Effects of Ephedrine on Histological Structure,Activities of Superoxide Dismutase,Catalase,and Content of Maleic Dialdehyde in Kidney of Filial Mice

作　　者：王锦锦[1] 李重阳[2] 俞诗源[1] 孙隽[1] 贾宗平[2] 雷蕾[1] 杨妍妮[1] 

机构地区：[1]西北师范大学生命科学学院,兰州730070 [2]甘肃政法学院,兰州7300701

出　　处：《动物学杂志》2011年第6期118-125,共8页Chinese Journal of Zoology

基　　金：国家自然科学基金项目(No.30370163);甘肃省自然科学基金项目(No.1107RJZA141);兰州市社会发展项目(No.2010-127);甘肃省证据科学技术研究与应用重点实验室科技计划项目(No.ZDSYS-Y08)

摘　　要：为了探讨麻黄素对仔鼠(Mus musculus)肾的毒性影响,对48只仔鼠分别用递增剂量腹腔连续注射麻黄素溶液(2.0、3.0、4.0 g/L)和等量的生理盐水,分别于注射5 d、10 d和15 d称量仔鼠体重和肾重,用比色法检测肾SOD、CAT活性及MDA含量的变化,用光学显微镜观察仔鼠肾组织结构的变化。结果表明,注射麻黄素5 d、10 d、15 d后仔鼠体重低于对照组,肾重高于对照组;仔鼠肾组织有不同程度的损伤,出现肾小球肿胀,肾小囊狭窄,肾小管和集合管上皮细胞肿胀、降解、坏死;肾SOD和CAT的活性先升高后降低,注射麻黄素5 d,SOD、CAT的活性高于对照组(P<0.05),注射麻黄素10 d、15 d,SOD和CAT的活性低于对照组(P<0.05或P<0.01);MDA含量则先降低后升高,注射麻黄素5 d,MDA含量低于对照组(P<0.05),注射麻黄素10 d、15 d,MDA含量高于对照组(P<0.05或P<0.01)。麻黄素影响仔鼠的体重和肾重,麻黄素对仔鼠肾的损伤可能与肾抗氧化酶活性降低和丙二醛含量升高有关。To study the poisonous effects of ephedrine on kidney,48 filial mice（Mus musculus） were intraperitoneally injected with escalation doses（2.0,3.0,4.0 g/L） of ephedrine or the same amount of saline.The samples were collected at 5,10 and 15 d respectively,and the body weight and kidney weight were measured,the activities of SOD and CAT as well as the content of MDA were detected by colorimetry.In addition,the changes of renal lesions were observed by optical microscopy.Results showed that the body weight of filial mice of the experimental group was lower than that of control group 5,10 and 15 d after ephedrine injection,while the kidney weight was higher than that of control group.Glomerular swelling and renal capsule narrowing were observed after injection of ephedrine.The epithelial cells of renal tubule and collecting tubule showed swelling,degeneration and necrosis.The activities of SOD and CAT were significantly higher 5 d after injection,while significantly lower 10 d and 15 d after injection when compared to the control.MDA content decreased in early stage and then increased.Thus,ephedrine significantly affects the body and kidney weight,and damages the kidney in filial mice,and the latter might be correlated with the decreased activity of SOD and CAT and the increased content of MDA.

关 键 词：麻黄素 仔鼠 肾 组织结构 SOD CAT MDA 

分 类 号：R285.5[医药卫生—中药学]