DEF对大豆蚜羧酸酯酶活性的抑制及其增效作用  

The effects of pretreatment with S,S,S-tributyl phosphorotrithioate on carboxylesterase activity and susceptibility to eight kinds of insecticides in the soybean aphid,Aphis glycines

作　　者：周正堂[1] 肖达[1] 卢延[1] 李锦钰[1] 董金锦[1] 宋敦伦[1] 高希武[1] 

机构地区：[1]中国农业大学农学与生物技术学院昆虫系,北京100193

出　　处：《应用昆虫学报》2011年第6期1669-1675,共7页Chinese Journal of Applied Entomology

基　　金：公益性行业(农业)科研专项(201103022)

摘　　要：对采自田间的大豆蚜Aphis glycines Matsumura在室内不接触任何药剂的情况下连续饲养30代以上作为被测虫源,研究DEF(1,2,4-三丁基三硫磷酸酯)不同时间处理大豆蚜后对高效氯氰菊酯等8种药剂的增效作用,以及大豆蚜体内羧酸酯酶的活性变化。结果显示,高效氯氰菊酯对经DEF处理10h的大豆蚜杀虫活性最高;大豆蚜在经DEF预处理2h后其体内羧酸酯酶剩余活性逐步降低,10h时活性降到最低值(69.6%),随后逐步升高,到24h时接近未处理前水平;DEF预处理大豆蚜10h后对8种药剂的LC50与DEF和杀虫剂混用以及与未经DEF预处理使用单一药剂测定的LC50相比分别为:高效氯氰菊酯(0.294、0.613、0.814mg·L-1),溴氰菊酯(0.047、0.181、0.340mg·L-1),氧化乐果(91.025、144.882、207.999mg·L-1),马拉硫磷(78.212、147.546、141.912mg·L-1),吡虫啉(1.778、7.689、11.876mg·L-1),啶虫咪(0.814、5.931、9.581mg·L-1),灭多威(7.120、19.559、37.335mg·L-1),克百威(11.298、20.957、23.927mg·L-1)。数据表明,DEF对大豆蚜羧酸酯酶活性的抑制在10h时最强;经DEF预处理试虫后8种药剂的毒力较未经DEF处理和DEF与杀虫药剂混用时的毒力增加,这一结论为田间增效剂的合理使用和大豆蚜抗性治理提供了技术支持和理论依据。The effects of exposing aphids to S,S,S-tributyl phosphorotrithioate（DEF） on the effectiveness of eight kinds of insecticides and on aphid carboxylesterase activity,were investigated in Aphis glycines（Matsumura） .Aphids were collected in the field and reared on soybean seedlings in a laboratory for more than 30 generations.The results show that beta-cypermethrin had the highest insecticidal activity on soybean aphids that had been exposed to DEF for 10 h.The carboxylesterase activity of soybean aphids decreased gradually after exposure to DEF for 2 h,and reached its lowest level,69.6%,after 10 h.Activity gradually recovered after 24 h.The LC50 values of eight insecticides with three treatments;exposure to DEF,pesticides mixed with DEF and pesticides without DEF,were as follows;beta-cypermethrin（0.294,0.613,0.814 mg·L-1） ,deltamethrin（0.047,0.181,0.340 mg·L-1） ,omethoate（91.025,144.882,107.999 mg·L-1） ,malathion（78.212,147.546,141.912 mg·L-1） ,imidacloprid（1.778,7.689,11.876 mg·L-1） ,acetamiprid（0.814,5.931,9.581 mg·L-1） ,methomyl（7.120,19.559,37.335 mg·L-1） and carbofuran（11.298,20.957,23.927 mg·L-1） .The results suggest that the inhibitory effect of DEF on carboxylesterase was significantly greater in soybean aphids that had been exposed to DEF for 10 h.The pretreatment of soybean aphids with DEF may enhance insecticide activity compared to either using insecticides without such pretreatment,or applying a mixture of insecticide and DEF.Our results provide a theoretical basis for the chemical control of soybean aphids in the field.

关 键 词：大豆蚜 羧酸酯酶 DEF 杀虫剂 增效作用 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]