IgM抗体检测对肠道病毒71型感染者诊断价值的探讨  

Diagnostic value of detection of IgM antibodies to EV71-infection patients

作　　者：吴斌[1] 李亮[1] 汤奋扬[1] 祁贤[1] 祖荣强[1] 崔仑标[1] 朱凤才[1] 周明浩[1] 汪华[1] 

机构地区：[1]江苏省疾病预防与控制中心,南京210009

出　　处：《中华微生物学和免疫学杂志》2011年第10期934-937,共4页Chinese Journal of Microbiology and Immunology

基　　金：江苏省自然科学基金项目（BK2009434）

摘　　要：目的探讨IgM抗体检测对肠道病毒71型（EV71）感染哲的诊断价值，并与核酸检测和中和试验方法进行比较分析。方法采用EV71IgM抗体酶免检测试剂盒（EV71-IgM试剂）、EV71核酸检测试剂龠（EV71．PCR试剂）和I{l和试验方法，检测115例EV71感染者的EV71RNA、中和抗体和IgM抗体的水平，并进行比较性研究分析。结果对115例EV71感染者，EV71-lgM试剂对患者卣’份m清的检出率为80．9％（93／115，95％CI：72．5～87．6），对228份健康儿童血清，EV71-tgM试剂的枪出牢为2．6％。问时，对病发1～2d采集的54份血清检出率为70．4％（38／54），显示了较好的早期诊断价ffEi。EV71-PCR试剂对患者咽拭子标本的检出率为82．6％（95／115，95％CI：74．4-89．0），与EV71-IgM试剂棚比，差异无统计学意义。此外，EV71-IgM试剂与EV71-PCR试剂具有很好的H补性，两种方法联合使川呵提高检测灵敏度约10个百分点达到92．2％。结论EV71-IgM试剂具有良好的早期诊断价值，与EV71-PCR试剂联用可提高诊断率。Objective To evaluate the diagnostic value of detection of IgM antibodies to EV71-infection patients, and compared cbaraeterisation of RT-PCR, IgM capture ELISA and neutralization test. Methods Virus RNA, neutralization titer and IgM antibody in 115 EVTl-infection patients were detected by EV71 real-time RT-PCR kit（ EV7I-PCR kit）, neutralization test, and EV71 IgM-capture ELISA kit （EVTI-IgM kit） , respectively. Results Using EV71-IgM kit, the detection rate was 80.9% （93/115, 95% CI：72.5-87.6） among the 115 EV71-infection patients, and was 2.6% among the 228 healthy children. Simultaneously, sera collected after 1-2 day of disease onset showed an IgM positivity of 70.4% （38/ 54）. The positive rate of EV71-PCR among these patients was 82.6% （95/115,95% CI： 74.4-89.0） , so there was no statistically significant differences between it and EV71-IgM kit . In addition, the detection rate in EV71-infection patients could increase to 92.2% by combined detection of EVT1-PCR and EVTI-IgM kit. Conclusion EVTI-IgM kit was a rapid and valuable way for the early diagnosis of EVT1 infection, and could significantly improve detection rate for EV71 infection by combining with EV71-PCR kit.

关 键 词：肠道病毒71 IGM抗体 中和试验 实时荧光PCR 

分 类 号：R446[医药卫生—诊断学] R51[医药卫生—临床医学]