重组ACE2抑制人脐动脉平滑肌细胞前纤维蛋白-1表达及增殖  

Recombinant ACE2 inhibits Profilin-1 expression and cell proliferation in human umbilical arterial smooth muscle cells

作　　者：于茜[1] 钟久昌[2] 金海燕[3] 沈伟利[2] 叶佳颖[1] 郭俊明[1] 周细武[1] 

机构地区：[1]宁波大学医学院附属李惠利医院,浙江宁波315040 [2]上海交通大学医学院瑞金医院高血压研究所医学基因组学国家重点实验室上海市血管生物学重点实验室,上海200025 [3]上海交通大学医学院瑞金医院临床心理科,上海200025

出　　处：《基础医学与临床》2011年第12期1326-1329,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金(30973522;81170246);上海市浦江人才计划项目(11PJ1408300)

摘　　要：目的探讨重组血管紧张素转换酶2(ACE2)基因对血管平滑肌细胞前纤维蛋白-1(Profilin-1)表达及细胞增殖的影响。方法培养人脐动脉平滑肌细胞(HUASMC),用重组ACE2基因(rACE2)干预,进行血管紧张素Ⅱ(AngⅡ)刺激实验,分别用MTT法、实时定量PCR及Western blot检测HUASMC增殖与Profilin-1表达。结果与对照组相比,AngⅡ(100 nmol/L)刺激6 h后,HUASMC中Profilin-1表达明显增高(3.50±0.30 vs 1.00±0.10,P<0.05)。重组ACE2基因干预显著抑制上述增加(1.73±0.12 vs 3.50±0.30,P<0.05)。结论 ACE2基因过表达可明显逆转HUASMC中AngⅡ诱导的Profilin-1表达上调。Objective To investigate the effects of the recombinant angiotensin-converting enzyme 2 （rACE2） gene on Profilin-1 expression and cell proliferation in vascular smooth muscle cells. Methods The cultured human um- bilical arterial smooth muscle cells （HUASMC） were stimulated by Ang II in the presence or absence of rACE2 gene, HUASMC proliferation and Profilin-1 expression level were determined with MTF, real-time PCR and West- HUASMCs （3.50 ±0. 30 vs 1. 00 ±0. hours, which was inhibited by rACE2 10 ; n = 5 - 6, P 〈 0. 05 ） by stimulating with Ang 1I （ gene intervention （1.73 ±0. 12 vs 3.50 ±0. 30; n =5,6, 100 nmol/L）for 6 P 〈 0. 05）. Condusions ACE2 gene over-expression contributes to the reversion of the Ang II-induced Profilin-1 expression and proliferation in human umbilical arterial smooth muscle cells.

关 键 词：血管紧张素转换酶2 前纤维蛋白-1 高血压病 血管紧张素Ⅱ 细胞增殖 

分 类 号：R544.1[医药卫生—心血管疾病]