牛瑟氏泰勒虫二温式PCR检测方法的建立及应用  

Establishment and application of a two-temperature PCR assay for the detection of Theileria sergenti

作　　者：钱年超[1] 贾立军[1] 薛书江[1] 张影[1] 黄国明[1] 张守发[1] 

机构地区：[1]延边大学农学院动物医学系,吉林延吉133002

出　　处：《中国兽医科学》2011年第11期1150-1153,共4页Chinese Veterinary Science

基　　金：国家自然科学基金资助项目(30960278)

摘　　要：为建立一种能快速、特异、敏感地检测牛瑟氏泰勒虫的方法,根据GenBank中的牛瑟氏泰勒虫MPSP基因(GQ180198.1)设计合成了1对特异性引物,建立了检测牛瑟氏泰勒虫的二温式PCR方法。该方法与犬新孢子虫、猪支原体和弓形虫RH株均无交叉反应,能检测到的最低DNA含量为1.7pg/μL。结果表明,该二温式PCR检测方法具有较高的特异性和敏感性。本研究为牛瑟氏泰勒虫的快速检测提供了一种方法。To establish a rapid,specific and sensitive detection method for Theileria sergenti,a pair of specific primers was designed and synthesized according to the T.sergenti MPSP gene in GenBank（GQ180198.1）and a two-temperature PCR assay was established.The method showed no cross reaction with Neospora caninum,Mycoplasma suis and Toxoplasma gondii RH strain.The minimum detectable amount of DNA was 1.7pg/μL.The results showed that the PCR method was highly sensitive and speci-fic.This study provided a method for rapid detection of T.sergenti.

关 键 词：瑟氏泰勒虫 MPSP基因 二温式聚合酶链反应 

分 类 号：S852.723[农业科学—基础兽医学]