瘦素对人牙髓干细胞骨向分化作用的实验研究  

Effects of leptin on the osteogenic differentiation of human dental pulp stem cells

作　　者：王林 张清彬[2] 张兆强[2] 

机构地区：[1]湖北省襄阳市口腔医院正畸科,湖北襄阳441003 [2]广州医学院口腔医院口腔颌面外科,广东广州510140

出　　处：《中国美容医学》2011年第12期1909-1911,共3页Chinese Journal of Aesthetic Medicine

摘　　要：目的:探讨瘦素(leptin)对人牙髓干细胞(Human dental pulp stem cells,hDPSCs)增殖和骨向分化的影响。方法:采用酶消化法体外培养人牙髓干细胞,分别加阶梯浓度leptin处理,通过四唑盐(MTT)比色试验和碱性磷酸酶(Alkalinephosphatase,ALP)活性测试来检测瘦素对牙髓干细胞体外增殖分化的影响;再将人牙髓干细胞与羟基磷灰石/磷酸三钙材料制作为复合体植入免疫缺陷裸鼠,12周后采用组织学和免疫组化方法检测体内生成物。结果:瘦素对人牙髓干细胞细胞增殖无明显促进作用,但leptin能促进人牙髓干细胞碱性磷酸酶的活性表达;体内试验证明leptin可提高牙髓干细胞向成成牙本质细胞分化及生成类牙本质的能力。结论:瘦素对人牙髓干细胞的增殖活性无明显作用,但人牙髓干细胞与羟基磷灰石磷酸三钙制作为复合体,可明显促进人牙髓干细胞骨向分化。Objective To test the effects o f leptin on proliferation and osteogenic differentiation of human dental pulp stem cells.Methods hDPSCs were cultured with enzyme-digestion technique.Different concentration of leptin was added to the hDPSCs for 3 days,MTT assay was used to evaluate the effects of proliferation,as well as detection of ALP activity.Each cell group was incubated with hydroxyapatite/tricalcium phosphate（HA/TCP） carrier and then transplanted subcutaneously into the back of immunocompromised mice to investigate the different formation of in vivo calcification after 12 weeks.Results No obvious changes were observed in the proliferation of hDPSCs after incubation with leptin,however leptin increased the expression of ALP activity.Histological examination and immunohistochemical staining with anti-human mitochondria antibody showed that leptin-treated DPSCs generated more dentin-like structures.Conclusion On hand,Leptin showed almost no effects on the proliferation of hDPSCs.On the other hand,this study verified the ability of leptin on hDPSCs to enhance odontogenic/osteogenic differentiation.Thus it has provided evidence that leptin acts as an important modulator of dental MSCs differentiation.

关 键 词：瘦素 牙髓干细胞 增殖 羟基磷灰石/磷酸三钙 骨向分化 

分 类 号：Q813.1[生物学—生物工程]