稻瘟病菌菌株FJ81278中新发现的一个基因注释及其表达分析  

Annotation and expression analysis of a novel unigene in Magnaporthe oryzae isolate FJ81278

作　　者：翟焕趁[1] 连壁[2] 王杏杏[3] 王宝华[3] 鲁国东[3] 王宗华[1,3] 

机构地区：[1]福建农林大学生命科学学院 [2]福建农林大学计算机与信息学院 [3]福建农林大学植物保护学院,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2011年第6期570-575,共6页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家公益性行业计划(200803008);国家自然科学基金(30400260;3000111)

摘　　要：对稻瘟病菌菌株FJ81278进行基因组和转录组分析,发现一个新的特异基因MoHsbA编码的蛋白具有疏水表面结合蛋白A(HsbA)结构域和一个信号肽.进一步的生物信息学分析发现,该基因组中另外7个基因编码的蛋白也具疏水表面结合蛋白结构域.稻瘟病菌中的HsbA蛋白聚集于系统发育树的同一个大的分支上,说明它们具有较近的亲缘关系.转录组和EST数据库中HsbA的表达情况不同,但在菌株GUY11侵染阶段基因芯片中均表现为上调.实时定量PCR分析表明,MoHs-bA在菌株FJ81278不同生长发育阶段均有表达,但在附着胞阶段表达量相对较高,推测该基因与附着胞的形成相关.研究结果为进一步揭示稻瘟病菌HsbA的生物学功能奠定了基础.To clone novel unigenes in Magnaporthe oryzae isolate FJ81278,we analyzed its genome and transcriptome data and found a unigene MoHsbA encoding protein with hydrophobic surface binding domain（HsbA） and a potential signal peptide.Further bioinformatics analysis showed that there were other 7 genes encoding proteins with HsbA domain.Interestingly,all these HsbA proteins were clustered in the same clade of the phylogenetic tree,suggesting they had close relation.In the transcriptome and EST database of M.oryzae,the HsbA genes had a diverse expression profiles,but they were all up-regulated genes based on microarray data of the isolate GUY11.However,our qRT-PCR results showed that MoHsbA expressed during all the growth and development stages,but with relative higher transcript level during appressorial development.Taking together,results will facilitate the understanding of the function of HsbA genes in M.oryzae.

关 键 词：稻瘟病菌 疏水表面结合蛋白A 转录组 表达分析 

分 类 号：Q933[生物学—微生物学]