拟南芥CYP76C2基因(At2g45570)的克隆及其菌核病抗性功能分析  

Cloning of Arabidopsis thaliana CYP76C2(At2g45570) and analysis of its resistance to Sclerotinia sclerotiorum

作　　者：陈晓婷[1,2] 孙翠霞[1,2] 王爱荣[2,3] 鲁国东[2,3] 

机构地区：[1]福建农林大学生命科学学院 [2]福建农林大学功能基因组研究中心 [3]福建农林大学植物保护学院,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2011年第6期606-609,共4页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金项目(30671347);公益性行业(农业)科研专项(nyhyzx07-05);中国博士后基金面上资助项目(20100480710);福建农林大学博士后项目(80254)

摘　　要：以拟南芥基因组DNA为模板,扩增出细胞色素P450(CYP76C2)基因的ORF,成功构建了CYP76C2基因的过表达载体并获得转基因植株.通过离体和活体接种表明,CYP76C2基因的过表达植株对核盘菌的抗性增强.Cytochrome P450（CYP76C2） was amplified using Arabidopsis genomic DNA as template,over-expression vectors of CYP76C2 were successfully constructed and transgenic plants were obtained.The over-expression CYP76C2 plants showed significant resistance to Sclerotinia sclerotiorum in detached leaves and in vivo.

关 键 词：拟南芥 细胞色素P450 菌核病 草酸 

分 类 号：S432.4[农业科学—植物病理学]