低强度脉冲超声波对Beagle犬下颌第三前磨牙慢性牙周炎组织学效应的探讨  

A Research of Chronic Periodontitis Histological Effects Upon the Third Premolars of Beagles' Mandibular with Low-intensity Pulsed Ultrasound

作　　者：刘婷[1] 宋锦璘[1] 刘珉懿[1] 王智彪[2] 赵纯亮[2] 邓锋[1] 

机构地区：[1]重庆医科大学附属口腔医院冉家坝院区正畸科,重庆市401147 [2]重庆医科大学生物医学工程系.重庆市生物医学工程重点实验室

出　　处：《中国超声医学杂志》2011年第11期970-973,共4页Chinese Journal of Ultrasound in Medicine

基　　金：国家自然科学基金(No.30870754);重庆市发展和改革委员会高技术产业重大产业技术开发项目(渝发改技No.[2007]1110);重庆卫生局科研项目(No.2008-2-254)

摘　　要：目的探讨低强度脉冲超声波(low-intenslty pulsed ultrasoumd,LIPUS)处理慢性牙周炎组织的生物学效应。方法 4只雄性Beagle犬构建下颌第三前磨牙慢性牙周炎模型,自体左右随机对照,不同强度LIPUS[超声功率(I_(SATA))30、60 mW/cm^2]连续3个月隔天处理实验组20min,每隔15 d测量牙龈表面温度,测量LIPUS处理前后牙槽骨骨密度并获取实验区域骨组织切片,经HE、Masson染色评估LIPUS处理下牙周炎组织改建效应。结果 LIPUS处理前后牙龈表面温度变化小于1℃。骨密度变化实验组>对照组(P<0.05);实验组I_(SATA) 30 mW/cm^2组<I_(SATA) 60 mW/cm^2组,无统计学差异(P>0.05)。不同参数LIPUS实验组较对照组均表现为牙槽骨组织成骨细胞活跃、类骨质形成增加。结论单纯LIPUS处理有减轻牙周炎症状的可能,对慢性牙周炎具有潜在的修复效应。Objective To explore the biological effect of chronic periodontitis with low intensity pulsed ultra sound（LIPUS） treatment solely. Methods Models including four male beagles were used in vivo to investigate issues relevant to the chronic periodontitis of third premolars. Canines were randomly divided into 2 groups to receive ultra- sound therapy of different intensities（IsAzA 30 mW/cm2, ISATA 60 mW/cm2）, and randomly separate bilateral sides of each dog into two groups： the experimental groups with LIPUS irradiation and control groups without opening power source of LIPUS radiation. LIPUS treatments were given for 20 minutes with interval of one day during the consecu tive 3 months. The temperature of the Gingive^s surface was tested every 15 days and the bone mineral density were measured while tissue sections were used to assess the histological effects. Results The difference of gingivers surface temperature was less than 1℃ between before and after LIPUS irradiation. The changes of BMD in the experimental groups were more than those of control groups（P〈0.05）, so did the groups of ISATA 30 mW/cm2 and groups of ISATA 60 mW/cm2, unfortunately there was no statistical significant （P〉0.05）. It indicated an activation of osteoplast in alveolar tissue and the osteoid formation enhancement. Conclusions Sole LIPUS treatment may anesis the symptoms of chronic periodontitis and has potential to restore.

关 键 词：低强度脉冲超声波 BEAGLE犬 慢性牙周炎 修复 

分 类 号：R445.1[医药卫生—影像医学与核医学] R781.4[医药卫生—诊断学]